Phospho-SAE1 (Ser185) Polyclonal Antibody, Rockland™

Catalog No.	Quantity
50-199-1990	100 μg
50-199-1991	25 μL

Catalog No. 50-199-1990 Supplier Rockland Immunochemicals Supplier No. 600401B24

Description

Rabbit Polyclonal Antibody

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. This purified antibody is directed against human SUMO Activating Enzyme E1 protein. This antibody is specific for human SAE1 protein phosphorylated at S185. A BLAST analysis using the sequence of the immunizing peptide was used to suggest that this antibody would react with SUMO Activating Enzyme E1 protein from human (100%), bovine, dog, chimpanzee (96%), mouse (93%), and rat (92%) based on a high degree of sequence homology. Cross reactivity against this protein from other sources has not been determined.

Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2. form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999).

Specifications

• Antigen: Phospho-SAE1 (Ser185)
• Applications: ELISA, Western Blot
• Classification: Polyclonal
• Concentration: 1.02 mg/mL
• Conjugate: Unconjugated
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Gene: SAE1
• Gene Accession No.: A2VE14, Q6AXQ0, Q9R1T2, Q9UBE0
• Gene Alias: 2400010M20Rik; 2610044L12Rik; activator of SUMO1; AL033372; AOS1; AW743391; D7Ertd177e; FLJ3091; HSPC140; SAE1; sentrin/SUMO-activating protein AOS1; SUA1; SUMO-1 activating enzyme E1 N subunit; SUMO1 activating enzyme subunit 1; SUMO-1 activating enzyme subunit 1; SUMO-activating enzyme subunit 1; SUMO-activating enzyme subunit 1, N-terminally processed; ubiquitin-like (sentrin) activating enzyme E1A; ubiquitin-like 1 (sentrin) activating enzyme E1A; ubiquitin-like 1 (sentrin) activating enzyme subunit 1; Ubiquitin-like 1-activating enzyme E1A; ubiquitin-like protein SUMO-1 activating enzyme; Ubl1a1; Uble1a
• Gene Symbols: SAE1
• Host Species: Rabbit
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 10055, 308384, 476425, 505512, 56459
• Target Species: Bovine, Canine, Human, Mouse, Rat
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid