Invitrogen™ Phospho-SMAD2/SMAD3 (Ser465, Ser467, Ser423, Ser425) Recombinant Rabbit Monoclonal Antibody (ST0493)

Catalog No.	Quantity
MA556386	100 μL

Catalog No. MA556386 Supplier Invitrogen™ Supplier No. MA556386

Description

Rabbit Recombinant Monoclonal Antibody

Positive Control: A549 treated with 5 ng/mL TGF-beta1 for 24 hours whole cell lysate, C2C12 treated with 5 ng/mL TGF-beta1 for 24 hours whole cell lysate, HeLa starved overnight then treated with 10 ng/mL TGF-beta1 for 30 minutes cell lysate, NIH/3T3 starved overnight then treated with 10 ng/mL TGF-beta1 for 30 minutes cell lysate, A549, human large intestine tissue, mouse skin tissue, mouse kidney tissue. Tissue Specificity: Low tissue specificity. Subcellular Location: Cytoplasm, Nucleus. Sequence Similarities: 100% Mouse. Predicted band size: 48 kDa.

Smad proteins, the mammalian homologs of the Drosophila mothers against decapentaplegic (Mad), have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP-2 and BMP-4 function, while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and Activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to Activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad proteins.

Specifications

• Antigen: Phospho-SMAD2/SMAD3 (Ser465, Ser467, Ser423, Ser425)
• Applications: Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
• Classification: Recombinant Monoclonal
• Clone: ST0493
• Concentration: 1.07 mg/mL
• Conjugate: Unconjugated
• Formulation: TBS with 0.05% BSA, 40% glycerol and 0.05% sodium azide; pH 7.4
• Gene: SMAD2
• Gene Accession No.: P84022, Q15796, Q62432, Q8BUN5
• Gene Alias: 7120426M23Rik; AU022421; hMAD-2; hMAD-3; hSMAD2; hSMAD3; HSPC193; HsT17436; JV15-2; JV18; JV18-1; LDS1C; LDS3; MAD (mothers against decapentaplegic, Drosophila) homolog 3; MAD homolog 2; MAD homolog 3; mad homolog JV15-2; mad protein homolog; MAD, mothers against decapentaplegic homolog 3; mad3; MADH2; MADH3; MADR2; mad-related protein 2; mMad2; mMad3; mother against DPP homolog 2; mothers against decapentaplegic homolog 2; Mothers against decapentaplegic homolog 3; mothers against DPP homolog 2; mothers against DPP homolog 3; pSMAD2; Sma- and Mad-related protein 2; SMA- and MAD-related protein 3; SMAD; Smad 2; Smad 3; SMAD family member 2; SMAD family member 3; SMAD, mothers against DPP homolog 2; SMAD, mothers against DPP homolog 3; Smad2; Smad-2; Smad3
• Gene Symbols: SMAD2, SMAD3
• Host Species: Rabbit
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser423 and 425 of human Smad3.
• Purification Method: Protein A
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 17126, 17127, 4087, 4088
• Target Species: Human, Mouse
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG