Invitrogen™ Phospho-SMAD2/SMAD3 (Thr8) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA536028

Catalog No. PIPA536028

Description

This antibody detects endogenous protein at a molecular weight of 55 and 60 kDa. Purity is >95% by SDS-PAGE.

Smad proteins, the mammalian homologs of the Drosophila mothers against decapentaplegic (Mad), have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP-2 and BMP-4 function, while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and Activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to Activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad proteins.

Specifications

• Antigen: Phospho-SMAD2/SMAD3 (Thr8)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: SMAD2
• Gene Alias: 7120426M23Rik; AU022421; hMAD-2; hMAD-3; hSMAD2; hSMAD3; HSPC193; HsT17436; JV15-2; JV18; JV18-1; LDS1C; LDS3; MAD (mothers against decapentaplegic, Drosophila) homolog 3; MAD homolog 2; MAD homolog 3; mad homolog JV15-2; mad protein homolog; MAD, mothers against decapentaplegic homolog 3; mad3; MADH2; MADH3; MADR2; mad-related protein 2; mMad2; mMad3; mother against DPP homolog 2; mothers against decapentaplegic homolog 2; Mothers against decapentaplegic homolog 3; mothers against DPP homolog 2; mothers against DPP homolog 3; pSMAD2; Sma- and Mad-related protein 2; SMA- and MAD-related protein 3; SMAD; Smad 2; Smad 3; SMAD family member 2; SMAD family member 3; SMAD, mothers against DPP homolog 2; SMAD, mothers against DPP homolog 3; Smad2; Smad-2; Smad3
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 17126, 17127, 25631, 29357, 4087, 4088
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunoprecipitation, Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: O70436, P84022, P84025, Q15796, Q62432, Q8BUN5
• Gene Symbols: SMAD2, SMAD3
• Immunogen: Synthetic phosphopeptide derived from human Smad2/3 around the phosphorylation site of Threonine 8.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov