Invitrogen™ Human SMAD3 (Phospho) [pS423/pS425] InstantOne™ ELISA Kit

Description

ELISA

The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated human SMAD3 (Ser423/425) in cell lysates. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate. Suitable Sample Types Tested on cell culture samples. Expected to work on many other sample types, but have not been tested at this time.

SMAD3 belongs to the SMAD family of proteins. They are similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.

Specifications

• Accession Number: P84022
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Cell Lysates
• Target Species: Human
• For Use With (Application): ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 4088
• Gene Symbol: HSPC193,HsT17436,JV15-2,LDS1C,LDS3,MADH3
• Kit Contents: Capture Antibody, Detection Antibody Reagent, InstantOne™ Assay Plate, Cell Lysis Buffer, Enhancer Solution, Wash Buffer (10X), Substrate Reagent (TMB), Stop Solution, Positive Control Cell Lysate (lyophilized), Plate seal
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 20 μL
• Storage Requirements: 2°C to 8°C
• Synonym: Mothers against decapentaplegic homolog 3
• Target: SMAD3
• Test Time: 1 hrs 20 mins
• Total Assay Time: 1 hrs 10 mins

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov