Phospho-STAT6 (Tyr641) Monoclonal Antibody (CHI2S4N), PE, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-112-2405	Invitrogen™ 12901342	100 Tests

Description

Description: This CHI2S4N monoclonal antibody recognizes human and mouse signal transducer and activator of transcription 6 (STAT6) when phosphorylated on tyrosine 641. Following their phosphorylation by JAKs, STAT proteins translocate to the nucleus where they bind to DNA and regulate transcription of specific genes in a cell type- and cytokine-specific manner. In response to IL-4, STAT6 is phosphorylated on tyrosine 641 by JAK1 and JAK3. STAT6 signaling downstream of the IL-4 receptor promotes T cell growth and B cell production of IgE. Specificity of this CHI2S4N clone was determined by ELISA and flow cytometry. Applications Reported: This CHI2S4N antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This CHI2S4N antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Staining Protocol: We recommend using Protocol C: Two-step protocol: Fixation/Methanol. Protocol A: Two-step protocol: intracellular (cytoplasmic) proteins and Protocol B: One-step protocol: intracellular (nuclear) proteins cannot be used.

STAT6 protein is a transcription factor activated by cytokines, particularly interleukin-4 and IL13. (Stat6-/-) were found to be deficient in IL-4-mediated functions including Th2 helper T-cell differentiation, expression of cell surface markers, T-cell proliferation, immunoglobulin class switching to IgE, and partial loss of IL-4 mediated proliferation. STAT6 mRNA has been detected in peripheral blood lymphocytes, colon, intestine, ovary, prostate, thymus, spleen, kidney, liver, lung and placenta. STAT6 is critically involved in Th2 immune response. STAT6 is crucial for IL-4-mediated biological responses, and naturally in two isoforms: STAT6a and STAT6c.

Specifications

• Antigen: Phospho-STAT6 (Tyr641)
• Concentration: 5 μL/Test
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene Accession No.: P42226, P52633
• Gene Symbols: STAT6
• Primary or Secondary: Primary
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Gene: STAT6

• Clone: CHI2S4N
• Applications: Flow Cytometry
• Conjugate: PE
• Host Species: Mouse
• Target Species: Human, Mouse
• Gene ID (Entrez): 20852, 6778
• Isotype: IgG2a κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

For Research Use Only.