Invitrogen™ Phospho-Tau (Ser202, Thr205) Monoclonal Antibody (AH36), APC

Catalog No.	Quantity
PIMA545933	100 μg

Catalog No. PIMA545933 Supplier Invitrogen™ Supplier No. MA545933

Description

Rabbit Monoclonal Antibody

A 1:500 dilution of MA5-45933 was sufficient for detection of Tau in 10 μg Baculovirus by dot blot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody. Detects approximately 79 kDa.

Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerization and stabilize microtubules. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton while the longer isoforms may preferentially play a role in its stabilization. In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer's diseases (AD) brain. Hyper-phosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases phosphorylate Tau, including GSK-3beta, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II. Hyper-phosphorylated Tau is found in neurofibrillary lesions in a range and other central nervous system disorders such as Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.

Specifications

• Antigen: Phospho-Tau (Ser202, Thr205)
• Applications: Dot Blot, ELISA, Immunohistochemistry, Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: AH36
• Concentration: 1 mg/mL
• Conjugate: APC
• Formulation: 2.48mM MES, 95.64mM phosphate with 0.5M EDTA and no preservative; pH 7.4
• Gene: MAPT
• Gene Accession No.: P10636, P10637
• Gene Alias: AI413597; AW045860; DDPAC; FLJ31424; FTDP17; FTDP-17; G protein beta1/gamma2 subunit-interacting factor 1; map tau; Mapt; MAPTL; MGC138549; microtubule associated protein tau; microtubule-associated protein tau; microtubule-associated protein tau, isoform 4; microtubules; MSTD; Mtapt; MTBT1; MTBT2; Neurofibrillary tangle protein; neurofibrillary tangles; Neuronal Marker; paired helical filament-tau; PHFtau; PHF-tau; PPND; PPP1R103; protein phosphatase 1, regulatory subunit 103; pTau; RNPTAU; Tau; Tau microtubule-associated protein; tau protein; Tau-4; Tau5; Unknown (protein for MGC:134287)
• Gene Symbols: MAPT
• Host Species: Rabbit
• Immunogen: Synthetic peptide of Human Phospho Tau (Ser202/Thr205).
• Purification Method: Affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 17762, 4137
• Target Species: Human, Mouse
• Content And Storage: 4°C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG

Safety and Handling

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