Invitrogen™ Phospho-VEGF Receptor 2 (Tyr1054, Tyr1059) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ 441047G

Catalog No. 44-104-7G

Description

On western blotting this antibody detects a protein with the apparent MW of 150 kDa. Positive controls used Porcine aortic endothelial (PAE) cells transfected with a chimeric receptor consisting of the extracellular domain of the CSF-1 receptor coupled to the transmembrane and cytoplasmic domains of the mouse VEGFR2, or 293T cells transfected with full length VEGFR2 and stimulated with 10 ng/mL VEGF for 5 minutes. VEGFR3 has not been tested, but is expected to react with this antibody.

KDR (FLK1, VEGFR2) is a type III receptor tyrosine kinase. It binds vascular endothelial growth factor with high affinity in vitro, and is expressed early in development by endothelial cell precursors. FLK1 (VEGF Receptor 2) encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc. Mutations of FLK1 (VEGF Receptor 2) are implicated in infantile capillary hemangiomas.

Specifications

• Antigen: Phospho-VEGF Receptor 2 (Tyr1054, Tyr1059)
• Classification: Polyclonal
• Formulation: Dulbecco's PBS with 1mg/mL BSA and 0.05% sodium azide; pH 7.3
• Gene Accession No.: P35918, P35968
• Gene Symbols: KDR
• Immunogen: The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human VEGFR2 that contains tyrosines 1054 and 1059. The sequence is conserved among multiple species including mouse and rat.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG

• Applications: Flow Cytometry, Western Blot, Immunocytochemistry
• Conjugate: Unconjugated
• Gene: KDR
• Gene Alias: 6130401C07; CD309; CD309 antigen; fetal liver kinase 1; fetal liver kinase-1; FLK1; Flk-1; FLK1 kinase insert domain receptor (a type III receptor tyrosine kinase) (VEGF receptor 2); FLK1 kinase insert domain receptor (VEGF receptor 2); flk-1 type VEGF receptor; KDR; kinase insert domain protein receptor; kinase insert domain receptor; kinase insert domain receptor (a type III receptor tyrosine kinase); kinase NYK; Krd-1; Ly73; protein-tyrosine kinase receptor flk-1; sCD309; soluble CD309; soluble vascular endothelial growth factor receptor 2; soluble VEGFR 2; soluble VEGFR2; sVEGFR-2; tyrosine kinase growth factor receptor; vascular endotheial growth factor 2; vascular endothelial growth factor receptor 2; vascular endothelial growth factor receptor- 2; vascular endothelial growth factor receptor-2; vascular endothelial growth factor receptor-3; VEGF R2; VEGF receptor-2; VEGFR; VEGFR2; VEGFR-2
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 16542, 3791
• Content And Storage: -20°C
• Form: Liquid