Invitrogen™ Phosphoserine/threonine Monoclonal Antibody (7A1P3)

Catalog No.	Quantity
PIMA538234	100 μL

Catalog No. PIMA538234 Supplier Invitrogen™ Supplier No. MA538234

Description

Mouse Monoclonal Antibody

Positive Samples: Jurkat.

A hallmark of signal transduction pathways is the reversible phosphorylation of serine and threonine residues within specific sequences, or motifs, in target proteins. Specific signaling motifs include not only sequences that are recognized by protein kinases, but also those that are recognized by phosphorylation-dependent binding proteins like 14-3-3. These modular phosphoprotein interacting domains are critical elements in modulating, directing and amplifying intracellular communications. Many critical protein kinases can be regulated by phosphorylation at a specific serine or threonine surrounded by phenylalanine or tyrosine. For example, Akt, an important kinase that regulates cell survival, is activated by phosphorylation at Ser473, a site surrounded by phenylalanine and tyrosine. RSK1, p70 S6 K, and certain PKC isoforms also contain a similar consensus phosphorylation site. Phosphorylation of these sites is required for kinase activity. The (Ser/Thr) Phe Antibody is a powerful tool for discovery of new proteins containing this important regulatory motif.

Specifications

• Antigen: Phosphoserine/threonine
• Applications: ELISA, Immunohistochemistry (Paraffin), Western Blot
• Classification: Monoclonal
• Clone: 7A1P3
• Concentration: 1.2 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 50% glycerol and 0.09% sodium azide; pH 7.3
• Host Species: Mouse
• Immunogen: A synthetic peptide corresponding to a sequence containing phosphorylated Serine/Threonine.
• Purification Method: Affinity chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Chemical, Human, Mouse, Rat
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2b κ
• Target Molecule: Chemical