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Invitrogen™ Phosphotyrosine Monoclonal Antibody (M375)
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Catalog No. PIMA547634
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100 μL
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PIMA547634 100 μL
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Catalog No. PIMA547634 Supplier Invitrogen™ Supplier No. MA547634
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Description

Mouse Monoclonal Antibody

This antibody detects phosphotyrosine-containing proteins on SDS-PAGE immunoblots of EGF treated A431 cells, as well as pervanadate treated A431, HeLa, Jurkat, and endothelial cells.

The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine -phosphorylated proteins and the biochemical pathways in which they function. The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Phosphotyrosine
Applications Immunoprecipitation, Western Blot, Immunocytochemistry
Classification Monoclonal
Clone M375
Concentration 1 mg/mL
Conjugate Unconjugated
Formulation PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
Gene NOS3
Gene Accession No. P29474, P70313, Q62600
Gene Alias Phosphotyrosine; pTyr; pY
Host Species Mouse
Immunogen Clone M375 was generated from tyrosine phosphorylated proteins purified from pervanadate treated A431 cells.
Purification Method Protein A
Quantity 100 μL
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 18127, 24600, 4846
Target Species Chemical, Mouse, Rat
Content And Storage -20°C, Avoid Freeze/Thaw Cycles
Product Type Antibody
Form Liquid
Isotype IgG2b
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