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Invitrogen™ Phosphotyrosine Monoclonal Antibody (pY20), HRP
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Catalog No. 037720
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100 μg
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Catalog No. Quantity
03-772-0 100 μg
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Catalog No. 03-772-0 Supplier Invitrogen™ Supplier No. 037720
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Description

Mouse Monoclonal Antibody

03-7720 has been successfully used in ELISA and WB applications, and detects proteins which contain phosphorylated tyrosine residues and proteins containing phosphorylated threonine residues.

The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine -phosphorylated proteins and the biochemical pathways in which they function. The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest.
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Specifications

Antigen Phosphotyrosine
Applications ELISA, Western Blot
Classification Monoclonal
Clone pY20
Concentration 0.5 mg/mL
Conjugate HRP
Formulation PBS with 4mg/mL BSA, 40% glycerol and 0.19% Kathon™ CG; pH 7.4
Gene Alias Phosphotyrosine; pTyr; pY
Host Species Mouse
Immunogen Keyhole limpet hemocyanin coupled with equal amounts of phosphotyrosine, glycine, and alanine.
Purification Method Purified
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Chemical
Content And Storage 4°C
Product Type Antibody
Form Liquid
Isotype IgG2b
Target Molecule Chemical
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Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov
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