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Invitrogen™ Phosphotyrosine Polyclonal Antibody, HRP
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Catalog No. 615820
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100 μg
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Catalog No. Quantity
61-582-0 100 μg
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Catalog No. 61-582-0 Supplier Invitrogen™ Supplier No. 615820
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Description

Rabbit Polyclonal Antibody

Phosphotyrosine Polyclonal Antibody for Western Blot, ELISA

The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine -phosphorylated proteins and the biochemical pathways in which they function. The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest.
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Specifications

Antigen Phosphotyrosine
Applications ELISA, Western Blot
Classification Polyclonal
Concentration 1 mg/mL
Conjugate HRP
Formulation PBS with 4mg/mL BSA, 40% glycerol and 0.1% ProClin 300; pH 7.4
Gene Alias Phosphotyrosine; pTyr; pY
Host Species Rabbit
Immunogen Rabbit anti-Phosphotyrosine-HRP; HRP conjugated Polyclonal antibody specific to Multiple Phosphotyrosine [pY]. This antibody is validated for use in Western Blot, Immunoassay (ELISA).
Purification Method Affinity chromatography
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Chemical
Content And Storage 4°C
Product Type Antibody
Form Liquid
Isotype IgG
Target Molecule Chemical
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Frequently Asked Questions (FAQs)

Do preparations of Invitrogen HRP-conjugated antibodies contain sodium azide?

HRP is inhibited by sodium azide, so the HRP-conjugated antibodies do not contain this agent.

Unconjugated antibodies are, however, supplied in solutions containing sodium azide.

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov
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