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  7. Thermo Scientific™ Phusion Blood Direct PCR Kit

Thermo Scientific™ Phusion Blood Direct PCR Kit

Catalog No. F547S
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F547S 100 Reactions
F547L 500 Reactions
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Description

Thermo Scientific Phusion Blood Direct PCR Kit is developed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR.

Thermo Scientific Phusion Blood Direct PCR Kit is for use in the amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR. The modified Phusion Hot Start II High-Fidelity DNA Polymerase exhibits high resistance to PCR inhibitors found in blood and retains polymerase activity even with samples containing 40% whole blood.

This DNA polymerase exhibits exceptional accuracy in proofreading and makes the Phusion Blood Direct PCR Kit one of the best choices for demanding applications, such as cloning. The Phusion Blood Direct PCR Kit is versatile and permits analysis of blood samples from various species and with different anticoagulants or storage conditions.

Features

  • No need for time-consuming and expensive DNA purification steps
  • Simple protocol requires minimal hands-on time
  • Extremely short PCR protocol times
  • Modified Phusion Hot Start II High-Fidelity DNA Polymerase delivers abundant yields of specific product

Notes

  • The optimal annealing temperature for Phusion DNA Polymerases may differ significantly from that of Taq-based polymerases.
TRUSTED_SUSTAINABILITY

Specifications

Concentration 2 U/μL
Content And Storage • Phusion Blood II DNA Polymerase, 40 μL
• 2X Phusion Blood PCR Buffer with dNTPs and MgCl2, 1.25 mL
• Universal Control Primer Mix, 40 μL
• 50 mM EDTA, 0.5 mL
• 50 mM MgCl2, 1.5 mL
• DMSO, 0.5 mL

Store at -20°C.
GC-Rich PCR Performance High
PCR Method Direct PCR
Polymerase Phusion Blood II DNA Polymerase
Reaction Speed Fast
Product Type Direct PCR Kit
Quantity 100 reactions
Shipping Condition Wet or Dry Ice
Hot Start Built-In Hot Start
No. of Reactions 100 Reactions
Overhang Blunt
Reaction Format Separate Components
Sample Type Blood
Starting Material DNA
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For Research Use Only. Not for use in diagnostic procedures.

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