Thermo Scientific™ Phusion Blood Direct PCR Master Mix

Description

Thermo Scientific Phusion Blood Direct PCR Master Mix is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR.

Thermo Scientific Phusion Blood Direct PCR Master Mix is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR. The modified Phusion Hot Start II High-Fidelity DNA Polymerase is resistant to main PCR inhibitors present in blood and retains its activity at blood concentrations of up to 40% in the PCR reaction.

This accurate proofreading DNA polymerase makes the Phusion Blood Direct PCR Master Mix an ideal choice for demanding applications, such as cloning. Phusion Blood Direct PCR Master Mix also allows analysis of blood samples with different preservatives (e.g., heparin, EDTA, citrate), storage conditions, and from various species.

Highlights

• Direct PCR allows sample to be added directly to PCR reaction, reducing time-consuming and expensive DNA purification steps
• Available Dilution & Storage protocol allows several PCR reactions from one sample
• Specially engineered Phire Hot Start II DNA polymerase for extremely short PCR protocol times
• Master mix format with premixed gel loading dye minimizes risk of cross-contamination, reduces sample handling, and allows direct loading on gel

Applications

• Genotyping
• Transgene detection
• Knockout analysis
• Sequencing
• Cloning

Notes

• Annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases.

Specifications

• Concentration: 2X
• Content And Storage: • 2X Phusion Blood Direct PCR Master Mix, 1 mL
  • Universal Control Primer Mix, 40 μL
  • 50 mM EDTA, 0.5 mL
  • 50 mM DMSO, 0.5 mL
  • 50 mM MgCl₂, 1.5 mL
  • Nuclease-free water, 1.25 mL
  • O'GeneRuler Express DNA Ladder, 50 μg (sufficient for 100 applications)
  
  Store at -20°C.
• GC-Rich PCR Performance: High
• PCR Method: Direct PCR
• Polymerase: Phusion Blood II DNA Polymerase
• Reaction Speed: Fast
• Includes Label or Dye: Yes
• Product Type: Direct PCR Master Mix
• Quantity: 100 reactions
• Shipping Condition: Wet or Dry Ice
• For Use With (Application): Genotyping
• Hot Start: Built-In Hot Start
• No. of Reactions: 100 Reactions
• Overhang: Blunt
• Reaction Format: SuperMix or Master Mix
• Sample Type: Blood
• Starting Material: DNA

For Research Use Only. Not for use in diagnostic procedures.