Thermo Scientific™ Phusion™ HF and GC Buffer Packs

Catalog No.	Chemical Name or Material	Color
F539L	GC Buffer Pack	Green
F518L	HF Buffer Pack	Colorless
F519L	GC Buffer Pack	Colorless
F520L	Detergent-free FF Buffer Pack	Colorless
F521L	Detergent-free GC Buffer Pack	Colorless
F538L	HF Buffer Pack	Green

Catalog No. F539L Supplier Thermo Scientific™ Supplier No. F539L

Description

Includes

Phusion GC Buffer Pack

• 5X Phusion GC Buffer (1X buffer provides 1.5 mM MgCl²)
• 50 mM MgCl2
• DMSO

Phusion Green GC Buffer Pack

• 5X Phusion Green GC Buffer (1X buffer provides 1.5 mM MgCl₂)
• 50 mM MgCl₂
• DMSO

5X Phusion Green buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel.

The error rate of Phusion DNA Polymerase in HF Buffer (4.4 x 10^-7) is lower than that in GC Buffer (9.5 x 10^-7). Therefore, the HF Buffer should be used as the default buffer for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures. For applications such as microarray or DHPLC, where the DNA templates need to be free of detergents, we recommend using detergent-free reaction buffers.

5X Phusion Green Buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffers do not interfere with the enzyme performance and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Two optimized buffers are available for Thermo Scientific Phusion High-Fidelity DNA Polymerases:

• 5X Phusion HF Buffer (F-518L, F-538L (Phusion Green), F-520L (Detergent-free))
• 5X Phusion GC Buffer (F-519L, F-539L (Phusion Green), F-521L (Detergent-free))

Specifications

• Chemical Name or Material: GC Buffer Pack
• Color: Green
• For Use With (Application): Standard PCR
• Quantity: 4 x 1.5 mL
• Includes: • 5X Phusion Green GC Reaction Buffer (4 x 1.5 mL)
  • 50 mM MgCl₂ (4 x 1.5 mL)
  • DMSO (0.5 mL)
  
  Store at –20°C.

For Research Use Only. Not for use in diagnostic procedures.