Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerase & dNTP Mix (10 mM each)

Catalog No.	Quantity
F530N	2000 reactions

Catalog No. F530N Supplier Thermo Scientific™ Supplier No. F530N

Description

This package includes Phusion High-Fidelity DNA Polymerase (4 x 500 U) and high quality dNTP Mix (10 mM each, 2 x 1 mL).

Thermo Scientific Phusion High-Fidelity DNA Polymerase sets a high standard for high performance PCR. Featuring an error rate 52-fold lower than that of Taq and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Phusion DNA polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors and generate higher yields with lower enzyme amounts than other DNA polymerase.

Features

• High fidelity (52X Taq)
• Fast PCR due to short extension times (15-30 s/kb)
• Robust performance, minimal optimization needed
• High yields of PCR products with minimal enzyme amounts

Applications

• Standard PCR
• RT-PCR
• High fidelity and long PCR
• LAMP, RDA, and MDA
• cDNA synthesis
• qPCR
• DNA labeling and sequencing

dNTP Mix

Thermo Scientific dNTP Mix contains pre-mixed aqueous solutions of dATP, dCTP, dGTP, and dTTP, each at a final concentration of 10 mM. The nucleotides have greater than 99% purity and are free of nuclease activities, as well as human and E. coli DNA. Mixes offer the possibility of reducing the number of pipetting steps and the risk of reaction setup errors.

dNTP features

• Stable after multiple freeze-thaw cycles
• Up to 95% of dNTPs remain in triphosphate form after 7 weeks at room temperature
• Up to 90% of dNTPs remain in triphosphate form after 30 cycles of PCR (1 minute at 94°C, 3 minutes at 72°C)

Notes

• Annealing rules for Phusion DNA polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).

Specifications

• Concentration: 2 U/μL
• Content And Storage: • Phusion High-Fidelity DNA Polymerase (4 x 500 U at 2 U/μL)
  • 10 mM dNTP Mix (2 x 1 mL)
  • 5X Phusion HF & GC Buffers
  • DMSO
  • 50 mM MgCl₂ solution
  
  Store at –5°C to –30°C.
• Detection Method: Primer-probe
• GC-Rich PCR Performance: High
• PCR Method: 1-step RT-qPCR
• Polymerase: Phusion High Fidelity DNA Polymerase
• Reaction Speed: Fast
• Product Type: High-Fidelity DNA Polymerase
• Quantity: 2000 reactions
• Shipping Condition: Dry Ice
• For Use With (Application): Standard PCR, High-fidelity PCR
• Fidelity (vs. Taq): 52X
• Hot Start: No
• No. of Reactions: 2000 Reactions
• Overhang: Blunt
• Reaction Format: Separate Components
• Size (Final Product): 20 kb or less

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.