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Thermo Scientific™ Phusion High-Fidelity PCR Kit

Get superior performance for cloning and other applications requiring high fidelity with one of the most accurate thermostable DNA polymerases available.

Supplier:  Thermo Scientific™ F553L

 View more versions of this product

Catalog No. F553L


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Description

Description

Highlights

  • Extreme fidelity (error rate 4.4 x 10-7 in Phusion HF Buffer)
  • Polymerase speed allows short extension times (15 to 30 s/kb)
  • Robust performance, minimal optimization needed
  • Increased product yields with minimal enzyme amounts
  • Direct loading on gel with clear Buffer
TRUSTED_SUSTAINABILITY
Specifications

Specifications


• Phusion DNA Polymerase (2 U/μL)
• 5X Phusion GC Buffer
• 5X Phusion HF Buffer
• dNTP mix (10 mM each)
• MgCl2 solution (50 mM)
• Control template (λ DNA)
• 1.3 kb primers (4 μM each)
• 10 kb primers (4 μM each)
• DNA size standard
• DMSO

Store at -20°C.
High
Fast
High-Fidelity PCR Kit
Cloning
No
Blunt
20 kb
Kit
Phusion High-Fidelity DNA Polymerase
Phusion
200 x 50 μL Reactions (or 500 x 20 μL Reactions)
52 X
200 Reactions
Separate Components
DNA
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Recommendations

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