Thermo Scientific™ Phusion High-Fidelity PCR Kit
Get superior performance for cloning and other applications requiring high fidelity with one of the most accurate thermostable DNA polymerases available.
Supplier: Thermo Scientific™ F553L
- Extreme fidelity (error rate 4.4 x 10-7 in Phusion HF Buffer)
- Polymerase speed allows short extension times (15 to 30 s/kb)
- Robust performance, minimal optimization needed
- Increased product yields with minimal enzyme amounts
- Direct loading on gel with clear Buffer
• Phusion DNA Polymerase (2 U/µL)
• 5X Phusion GC Buffer
• 5X Phusion HF Buffer
• dNTP mix (10 mM each)
• MgCl2 solution (50 mM)
• Control template (λ DNA)
• 1.3 kb primers (4 µM each)
• 10 kb primers (4 µM each)
• DNA size standard
Store at -20°C.
|Phusion DNA Polymerase, 5x Phusion HF Buffer, 5x Phusion GC Buffer, dNTP Mix, MgCl2 solution, control lambda DNA, 1.3kb control primers, 10kb control primers, DNA size standard, DMS|
|High-Fidelity PCR Kit|
|Phusion High-Fidelity DNA Polymerase|
|200 x 50 μL Reactions (or 500 x 20 μL Reactions)|
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