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Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerases (2 U/μL)

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

$155.60 - $2123.00

Specifications

GC-Rich PCR Performance High
Reaction Speed Fast
Product Type High-Fidelity DNA Polymerase
Shipping Condition Dry Ice
For Use With (Application) Standard PCR, High-fidelity PCR
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Products 5
Catalog Number Mfr. No. Color No. of Reactions Price Quantity & Availability  
Catalog Number Mfr. No. Color No. of Reactions Price Quantity & Availability  
F530S
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Thermo Scientific™
F530S
Colorless 100 Reactions
Each for $167.00
Add to Cart
 
F530L
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Thermo Scientific™
F530L
Colorless 500 Reactions
Each for $655.00
Add to Cart
 
F530XL
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Thermo Scientific™
F530XL
Colorless 2000 Reactions
Each for $2,123.00
Add to Cart
 
F534S
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Thermo Scientific™
F534S
Green 100 Reactions
Each for $155.60
Edge
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F534L
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Thermo Scientific™
F534L
Green 500 Reactions
Each for $623.50
Add to Cart
 
Description

Description

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is an excellent choice for cloning and other applications requiring high fidelity. Phusion DNA polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerases.

Features of Phusion™ High-Fidelity DNA Polymerase include:

  • High fidelity (52X Taq)
  • Fast PCR due to short extension times (15–30 s/kb)
  • Robust performance, minimal optimization needed
  • High yields of PCR products with minimal enzyme amounts
  • Available in Green buffer format for direct loading of PCR products on gels (F-534S or F-534L)

Applications

  • High-fidelity PCR
  • Cloning
  • Template generation for sequencing
  • Amplification of difficult (GC-rich) templates
  • Long-range PCR (up to 20 kb)
  • Mutagenesis
  • High throughput PCR
  • Microarray

Note: Annealing rules for Phusion DNA polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our convenient Tm calculator.

Specifications

Specifications

High
High-Fidelity DNA Polymerase
Standard PCR, High-fidelity PCR
52X
Blunt
20 kb or less
Fast
Dry Ice
2 U/μL
No
Standalone
SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

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