Thermo Scientific™ Phusion Human Specimen Direct PCR Kit

Thermo Scientific Phusion Human Specimen Direct PCR Kit delivers unprecedented convenience for DNA amplification by allowing PCR directly from unpurified human samples.

Supplier:  Thermo Scientific™ F150BID

Catalog No. F150

Description

Thermo Scientific Phusion Human Specimen Direct PCR Kit delivers unprecedented convenience for DNA amplification by allowing PCR directly from unpurified human samples. A tiny amount of sample is used directly in the PCR reaction with no prior purification steps. This allows significant savings in both time and cost.

Tested sample materials include buccal swabs, saliva, amniotic fluid, hair, fingernails, teeth, and skin biopsies. The kit is compatible with fresh, frozen, and even formalin-fixed, paraffin-embedded (FFPE) human tissue samples. The Phusion Human Specimen Direct PCR Kit includes optimized reagents and detailed protocols for many sample materials. The modified Phusion Hot Start II High-Fidelity DNA Polymerase utilized in the kit is a highly accurate proofreading DNA polymerase, and PCR products obtained are perfectly suitable even for cloning purposes.

Features

• No need for time-consuming and expensive DNA purification steps
• Two simple protocols for various applications
• Extremely short PCR protocol times
• Modified Phusion Hot Start II High-Fidelity DNA Polymerase delivers abundant yields of specific product

Notes

• The optimal annealing temperature for Phusion DNA Polymerases may differ significantly from that of Taq-based DNA polymerases.

Specifications

• Content And Storage: • Phusion Human Specimen DNA Polymerase, 80 μL
  • 2X Phusion Human Specimen PCR Buffer, 2 x 1.25 mL
  • Universal Control Primer Mix (25 μM), 40 μL
  • Dilution Buffer, 2 x 5 mL
  • DNARelease Additive, 3 x 100 μL
  
  Store at -20°C.
• PCR Method: Direct PCR
• Reaction Speed: Fast
• Quantity: 200 reactions
• For Use With (Application): Hot-start PCR
• No. of Reactions: 200 reactions
• Reaction Format: Separate Components
• Starting Material: DNA

• GC-Rich PCR Performance: High
• Polymerase: Phusion Human Specimen DNA Polymerase
• Product Type: Direct PCR Kit
• Shipping Condition: Wet or Dry Ice
• Hot Start: Built-In Hot Start
• Overhang: Blunt
• Sample Type: Human

For Research Use Only. Not for use in diagnostic procedures.