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  6. Invitrogen™ Phycoerythrin Monoclonal Antibody (eBioPE-DLF), Biotin, eBioscience™ 

Invitrogen™ Phycoerythrin Monoclonal Antibody (eBioPE-DLF), Biotin, eBioscience™
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Catalog No. 501129375
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100 μg
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Catalog No. Quantity
50-112-9375 100 μg
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Catalog No. 50-112-9375 Supplier Invitrogen™ Supplier No. 13412082
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Description

Mouse Monoclonal Antibody

The eBioPE-DLF monoclonal antibody reacts with phycoerythrin (PE), PE-Cy5 and PE-Cy7, with no crossreactivity to fluorescein (FITC) or allophycocyanin (APC). When using eBioPE-DLF as a second-step antibody, in combination with an appropriate third step, to bind a PE-conjugated primary antibody, there is no decrease in the fluorescent intensity of the directly conjugated PE antibody. Therefore no interference or quenching of the PE fluorochrome is observed. The eBioPE-DLF can be used for separation of cells labeled with PE-conjugated antibodies or staining. Please note that the optimal concentration for this antibody will vary depending on the density of the primary PE-conjugated antibody and the application tested. Applications Reported: This eBioPE-DLF antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBioPE-DLF antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.06 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 μm post-manufacturing filtered.

Anti-RPE (R-Phycoerythrin) antibodies can be used to bind conjugated primary antibody without decreasing fluorescent intensity of the RPE fluorochrome. This method is used for separation of cells labeled with RPE-conjugated antibodies.
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Specifications

Antigen Phycoerythrin
Applications Flow Cytometry
Classification Monoclonal
Clone eBioPE-DLF
Concentration 0.5 mg/mL
Conjugate Biotin
Formulation PBS with 0.09% sodium azide; pH 7.2
Gene Alias PE; RPE; R-PE; R-phycoerythrin
Host Species Mouse
Purification Method Affinity chromatography
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Chemical
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Antibody
Form Liquid
Isotype IgG1 κ
Target Molecule Tag
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