Thermo Scientific™ Miser™ Antibody Extender Solution NC

Catalog No. PI32110 Supplier Thermo Scientific™ Supplier No. 32110

Description

Enable Western blot detection with much less primary antibody by treating nitrocellulose membrane with this reagent.

Perform Western blot detection with much less primary antibody using Thermo Scientific™ Pierce Antibody Extender Solution NC, a post-transfer nitrocellulose membrane-treatment reagent.

By treating nitrocellulose membranes for a few minutes in this reagent immediately after transfer and before probing, you can use much less (more dilute) primary antibody solutions while obtaining the same level of detection. This conserves use of expensive and rare antibodies, enabling more experiments to be performed with the same amount of antibody. The reagent is so effective that our scientists have observed antibody cost reductions of 10-, 25- and even 100-times (depending on the antigen and primary antibody directed against that antigen).

Highlights:

• Achieve equivalent signal – use at least three times less primary antibody than normal and retain comparable or better signal detectability

• Any detection mode or enzyme – the method works with both colorimetric and chemiluminescent detection modes and with horseradish peroxidase (HRP) and alkaline phosphatase (AP) systems

• Easy-to-perform, 10-minute protocol – invest a few minutes and save money on your primary antibody; our scientists have reduced their use of expensive primary antibodies by up to 100-fold with this product

Specifications

• Product Line: Miser
• Membrane: Nitrocellulose Membrane
• Form: Liquid
• Product Type: Antibody Extender Solution
• Content And Storage: Store at room temperature.
• For Use With (Application): ELISA, Western Blot

Frequently Asked Questions (FAQs)

Which should I use: the Pierce Western Blot Signal Enhancer or the Miser Antibody Extender Solution NC?

If you are happy with your western signal, you can use the Miser Antibody Extender Solution NC to minimize the amount of antibody you need to use and therefore reduce your costs. If you need to increase sensitivity, then you can use the Pierce Western Blot Signal Enhancer.

How does the Miser Antibody Extender Solution NC allow me to use less antibody?

The proteins on the blot become more accessible to the antibodies, making it possible for a signal to be produced using less antibody.

Can I use Ponceau S staining before using the Miser Antibody Extender Solution NC?

The Extender Solution should be used after staining with Ponceau S.

Can I use the Miser Antibody Extender Solution NC on nitrocellulose and PVDF membranes?

The Extender solution gives consistent, reproducible results on nitrocellulose membranes; results can vary with PVDF membrane.

When in my workflow do I need to used the Miser Antibody Extender Solution NC?

The Extender Solution should be used after transferring the protein to the membrane and before blocking the membrane.

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov