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Thermo Scientific™ Pierce™ Background Eliminator Kit

Rapidly remove haze and artifacts from X-ray film to correct overexposed blots, whether from chemiluminescent Western blot or DNA or RNA autoradiography.

Supplier:  Thermo Scientific™ 21065

Catalog No. PI-21065

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Thermo Scientific™ Pierce Background Eliminator provides fast, easy removal of haze and artifacts on developed X-ray film to correct for overexposure of blots, whether from chemiluminescent Western blotting or DNA or RNA autoradiography.

This two-reagent kit is simple to use. Mix the two reagents (1:1 ratio) and immerse a developed piece of film (recent or old) in the working solution. Watch for 1 to 5 minutes until the desired image appears, then stop the reaction by rinsing the film in water. Pierce Background Eliminator for Film evenly reverses development-signal over the entire film, preserving relative densitometry values so that analysis and publication of results is still valid and possible.


  • For overexposed X-ray film – reverses development of silver-based autoradiography film removing silver emulsion from the film surface

  • Recovers and preserved data – evenly reduces developed signal over the entire film so relative intensity (densitometry) of bands is not altered

  • Optimizes for signal-to-noise – allows readjustment of overdeveloped film to obtain the best image with respect to background haze, speckles and oversaturated bands that need to be resolved

  • Saves time and resources – eliminates the need to repeat experiments or re-expose a new piece of film to obtain an optimal image

Recommended for:

Remove background from any application that uses X-ray film exposures including: Western, Northern and Southern blots that use Thermo Scientific SuperSignal Substrates and the Pierce Chemiluminescent Hybridization and Detection Kit; In-gel detection systems; Gel-shift assays; Ribonuclease protection assays (RPA)



Background Eliminator Kit
Western Blot
Sufficient For: 2.5L of 1X solution to treat at least 10 films (13 x 18 cm ea.)
• Reagent A, 100 mL
• Reagent B, 100 mL

Store at room temperature
All species




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For Research Use Only. Not for use in diagnostic procedures.