Thermo Scientific™ BS3-d0 (bis(sulfosuccinimidyl) suberate-d0)

Description

Crosslink amines to amines using these heavy (deuterated) and light matched pairs of an 11.4A, NHS-ester crosslinker for mass spectrometry analysis of protein interactions.

Thermo Scientific™ Pierce BS3-d0/d4 reagents form a matched set of heavy (deuterated) and light mass varieties of the popular BS3 amine-reactive crosslinker that facilitates the identification of crosslinked peptides by mass spectrometry.

A new strategy to study protein structure and function is rapidly emerging that integrates the proven utility of crosslinking with the power of mass spectrometry (MS) to yield insights into protein tertiary structure and protein complex formation. The reagents introduced here will generate MS patterns in which the resultant crosslinked peptides will differ by 4 mass units after enzymatic digestion of the crosslinked protein or protein complex.

Highlights:

• Well-characterized, high-purity, deuterium-labeled crosslinker and its hydrogen-containing analog
• Instructions edited by an expert in the crosslinking/MS strategy field
• Suberate- and glutarate-based reagent pair - provides a “molecular ruler” option to study inter- and intra-molecular distances to gain structural information not possible with just one reagent
• Efficient and convenient - requires only microgram amounts of protein; an excellent alternative to NMR or X-ray crystallography-based methods that require large amounts of protein, special solvents or crystal formation
• Reactive groups: Sulfo-NHS ester (homobifunctional)
• Reactive toward: amino groups

Note:

References Illustrating Applications of Heavy/Light Crosslinking Reagents and Mass Spectrometry Sinz, A. (2003). Chemical crosslinking and mass spectrometry for mapping three-dimensional structures of proteins and protein complexes.J. Mass Spectrom.38, 1225-1237. Dihazi, G.H. and Sinz, A. (2003). Mapping low-resolution three-dimensional protein structure using chemical crosslinking and Fourier transform ion-cyclotron resonance mass spectrometry. Rapid Commun. Mass Spectrom.17, 2005-2014. Kalkhof, S.et al.(2005).

Specifications

• Chemical Reactivity: Amine-Amine
• Cleavable: No
• Description: BS3-d0
• Molecular Weight (g/mol): 572.43
• PEGylated: No
• Spacer Arm Length: 11.4 Å
• Content And Storage: Upon receipt store desiccated at 4 to 8°C.
• Cell Permeability: No
• Shipping Condition: Ambient
• Product Line: Pierce™
• Labeling Method: Chemical Labeling
• Crosslinker Type: Homobifunctional
• Reactive Moiety: Sulfo-NHS Ester
• Spacer: Medium (10 to 30 Å)
• Form: Powder
• Quantity: 10 mg
• Solubility: Water
• Format: Standard
• Water Soluble: Yes
• Product Type: Crosslinker

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.