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Thermo Scientific™ Pierce™ C18 Spin Tips & Columns

Thermo Scientific Pierce C18 Spin Columns (10–150 µL) and Spin Tips (10–100 µL) effectively remove interfering contaminants, while also maintaining excellent binding and recovery of peptide samples for use upstream of mass spectrometry and other high sensitivity detection methods.

Supplier:  Thermo Scientific™ 84850

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Catalog No. PI84850

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Matrix-assisted laser desorption ionization (MALDI) and electrospray ionization (ESI) mass spectrometry (MS) provide high sensitivity and mass accuracy; however, interfering molecules from the sample can result in reduced sensitivity and poor-quality spectra. Sample clean-up with C18 resins significantly improves signal-to-noise ratios and sequence coverage by removing MS-incompatible salts and detergents commonly used in protein or peptide preparation. The optimized protocol for the Pierce C-18 Spin Columns effectively removes contaminants and elutes peptides in MS-compatible solutions.

Pierce C18 Spin Columns
Thermo Scientific Pierce C18 Spin Columns are ready-to-use centrifuge columns of porous C18 reverse-phase resin for peptide sample preparation for MS and other analysis methods. The protocol has been optimized to efficiently remove interfering contaminants and release peptides in MS-compatible solutions. Columns can be used effectively for processing peptides derived from 10 ng to 30 µg of protein. Sensitivity and detection limits are dependent on the downstream application.

Efficient—effectively removes interfering contaminants to improve spectral quality and is suitable for a variety of reverse-phase-compatible contaminants
Flexible—works with a wide variety of load volumes and concentrations; no need to reduce sample volume before application
Convenient—spin column format requires no special equipment to process multiple samples simultaneously
Robust—special C18 resin allows excellent recovery percentages, even at low (sub-picomole) sample loads

Pierce C18 Spin Tips
Thermo Scientific Pierce C18 Spin Tips enable fast and efficient capture, concentration, desalting, and elution of up to 10 µg of peptides per 20 µL of sample with volumes ranging from 10 to 100 µL. The five-step procedure is simple and requires less than five minutes to process protein digests, strong cation exchange fractions, and other protein and peptide samples for mass spectrometric (MS) analyses. The Pierce C18 Spin Tips offer excellent flow properties with a high-efficiency monolithic C18 sorbent for fast wetting, loading, washing, and eluting.

Efficient—effectively removes interfering contaminants to improve spectral quality and is suitable for a variety of reverse-phase-compatible contaminants
Rapid—C18 fast-flow tips have low resistance and improved flow
Convenient—spin tips are provided with tip adaptor for easy centrifugation
Cleaner sample—device filters particulates that can cause autosampler and column clogging

• Clean-up of peptides after protein digestion
• Peptide sample concentration and desalting
• Sample preparation for MALDI peptide analysis and proteomics workflows



Microcentrifuge, Mass Spectrometer
Peptides, Protease-digested Protein
Mass Spectrometry
Sample Clean Up
Spin Tip
Store at room temperature.
96 Tips


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