Thermo Scientific™ Chloronaphthol Substrate Powder (4-chloro-1-naphthol)

Description

Powders, tablets and ready-made 4-chloro-1-naphthol solutions for chromogenic HRP detection in Western blot and tissue staining methods.

Thermo Scientific™ Pierce Chloronaphthol (4-CN) Substrates are powders, tablets and ready-made solutions of 4-chloro-1-naphthol for chromogenic detection of horseradish peroxidase (HRP) activity in blotting and tissue staining methods.

Chloronaphthol (4-CN, MW = 178.6) reacts with HRP to form a distinct blue to blue-purple product to localize and visualize the activity of peroxidase-conjugated antibodies on blots or fixed tissue samples. The HRP substrate is not as sensitive or stable as other chromogenic substrates, such as TMB and DAB. However, the blue-purple precipitate photographs well, and its alcohol-solubility is a property that can be utilized in certain double-staining applications.

Highlights:

• HRP substrate – for detection of horseradish peroxidase activity on solid media

• Chromogenic – no special equipment needed for visualization

• Blue-purple – easy to photograph in color or black-and-white

• Package options – choose powder, tablets, or ready-to-use solution

Specifications

• Product Type: Substrate
• Detection Method: Colorimetric
• Form: Powder
• Quantity: 25 g
• Substrate: Horseradish Peroxidase
• Content And Storage: Store at 4°C.