Thermo Scientific™ Pierce™ CN/DAB Substrate Kit

Description

Combines chloronaphthol- and diaminobenzidine-based HRP detection for Western blot and tissue staining methods.

Thermo Scientific™ Pierce CN/DAB Substrate Kit includes substrate and peroxide solutions for combined chloronaphthol- and diaminobenzidine-based detection of horseradish peroxidase (HRP) activity in blotting and tissue staining methods.

This kit combines two popular chromogenic peroxidase substrate compounds (4-CN and DAB) in a single, stable, 10X stock solution. This CN/DAB solution produces an intense dark black precipitate at sites of bound HRP-conjugated antibodies on probed blots and tissue samples. The Pierce CN/DAB Substrate Kit can be used in Western immunoblotting techniques as well as in dot blotting assays. The kit includes the 10X solution of CN/DAB and accompanying 1X Stable Peroxide Substrate Buffer.

Highlights:

• HRP substrate – for detection of horseradish peroxidase activity on solid media via the combined action of CN (4-chloro-1-naphthol) and DAB (3,3'-diaminobenzidine tetrahydrochloride)

• Chromogenic – no special equipment needed for visualization; yields intense black bands that are easy to photograph

• Easy-to-use kit – substrate stock solution and stable peroxide substrate buffer mean that there is no dry powders to measure and dissolve

• Stable – store refrigerated at 4°C for at least one year

Includes:

10X solution of CN/DAB and the Pierce Stable Peroxide Substrate Buffer

Specifications

• Product Type: Substrate
• Detection Method: Colorimetric
• Form: Liquid
• Product Line: Pierce™
• Quantity: 275 mL
• Conjugate: HRP
• Substrate: Horseradish Peroxidase
• Reactivity: Horseradish Peroxidase
• Sufficient For: 25 Uses at 10 mL Each
• Content And Storage: Sufficient For: 25 uses at 10 mL each
  • CN/DAB (10X), 25 mL
  • Stable Peroxide Buffer, 250 mL
  
  Store at 4°C.

For Research Use Only. Not for use in diagnostic procedures.