Thermo Scientific™ Pierce™ Controlled Protein-Protein Crosslinking Kit

Description

Chemically conjugate any two purified proteins (antibodies, enzymes, carriers, etc.) using sulfo-SMCC crosslinker, a complete kit with modification reagents and buffers.

Thermo Scientific™ Pierce Controlled Protein-Protein Crosslinking Kit contains essential crosslinkers, modification reagents and buffers for conjugating antibodies, enzymes and other purified proteins needed as probes.

The kit provides Sulfo-SMCC crosslinker for amine-to-sulfhydryl conjugation and SATA modification reagent for specific sulfhydryl-addition. The crosslinking strategy allows two purified proteins, such as an antibody and a reporter enzyme, to be conjugated in a two-step, controlled manner. Supplementary components, buffers and columns are also included to support successful creation of a useful conjugate via any one of several specific protocols.

Highlights:

• Sulfo-SMCC – high-quality crosslinker conjugates primary amines of protein 1 to sulfhydryl groups of protein 2

• SATA or 2-MEA – modification reagents provide two options for making sulfhydryl groups available on protein 2 (usually an antibody)

• Ellman's Reagent – provides a mean of monitoring and assessing efficiency of the final reaction

• Complete – includes critical reagents as well as buffers, desalting columns and supplementary modification reagents

Specifications

• Chemical Reactivity: Amine-Sulfhydryl
• Cleavable: No
• Description: Controlled Protein-Protein Crosslinking Kit
• Molecular Weight (g/mol): 436.37
• PEGylated: No
• Spacer Arm Length: 11.6 Å
• Content And Storage: Sufficient For: One conjugation with two purified proteins (up to 5 mg each)
  • Sulfo-SMCC Crosslinking Agent, 2 mg
  • Activation/Conjugation Buffer (10X), 20 mL
  • PBS packs (each makes 500mL), 2 packs
  • 2-Mercaptoethylamine-HCl, 6 mg
  • Immobilized-Reductant, 0.2 mL
  • SATA, 2 mg
  • Dimethylformamide (DMF), 1 mL
  • Hydroxylamine-HCl, 5 mg
  • Dextran Desalting Column, 2 x 10 mL
  • Column Extender, 2 ea.
  • Cysteine-HCl, 20 mg
  • Ellman's Reagent, 2 mg
  
  Store at 4°C.
• Cell Permeability: No
• Shipping Condition: Ambient
• Product Line: Pierce™
• Labeling Method: Chemical Labeling
• Crosslinker Type: Heterobifunctional
• Reactive Moiety: Maleimide, Sulfo-NHS Ester
• Spacer: Short (<10 Å)
• Form: Kit
• Quantity: 1 Reaction
• Solubility: Water
• Format: Kit
• Water Soluble: Yes
• Product Type: Crosslinker

For Research Use Only. Not for use in diagnostic procedures.