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Thermo Scientific™ Pierce™ Controlled Protein-Protein Crosslinking Kit
Description
Thermo Scientific™ Pierce Controlled Protein-Protein Crosslinking Kit contains essential crosslinkers, modification reagents and buffers for conjugating antibodies, enzymes and other purified proteins needed as probes.
Highlights:
Sulfo-SMCC – high-quality crosslinker conjugates primary amines of protein 1 to sulfhydryl groups of protein 2
SATA or 2-MEA – modification reagents provide two options for making sulfhydryl groups available on protein 2 (usually an antibody)
Ellman's Reagent – provides a mean of monitoring and assessing efficiency of the final reaction
Complete – includes critical reagents as well as buffers, desalting columns and supplementary modification reagents

Specifications
Specifications
Chemical Reactivity | Amine-Sulfhydryl |
Cleavable | No |
Description | Controlled Protein-Protein Crosslinking Kit |
Molecular Weight (g/mol) | 436.37 |
PEGylated | No |
Spacer Arm Length | 11.6 Å |
Content And Storage | Sufficient For: One conjugation with two purified proteins (up to 5 mg each) • Sulfo-SMCC Crosslinking Agent, 2 mg • Activation/Conjugation Buffer (10X), 20 mL • PBS packs (each makes 500mL), 2 packs • 2-Mercaptoethylamine-HCl, 6 mg • Immobilized-Reductant, 0.2 mL • SATA, 2 mg • Dimethylformamide (DMF), 1 mL • Hydroxylamine-HCl, 5 mg • Dextran Desalting Column, 2 x 10 mL • Column Extender, 2 ea. • Cysteine-HCl, 20 mg • Ellman's Reagent, 2 mg Store at 4°C. |
Cell Permeability | No |
Shipping Condition | Ambient |
Product Line | Pierce™ |
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For Research Use Only. Not for use in diagnostic procedures.
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