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Thermo Scientific™ Pierce™ ECL Plus Western Blotting Substrate

Catalog No. FER32132X3
Click to view available options
Quantity:
100 mL Kit
300 mL Kit
Sufficient For:
1000 cm2 of membrane
3000 cm2 of membrane

Acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.

Thermo Scientific™ Pierce ECL Plus Substrate is an acridan-based chemiluminescent and chemifluorescent substrate to detect peroxidase activity from HRP-conjugated probes by Western blotting using X-ray film, CCD or laser-based imagers.

Thermo Scientific Pierce ECL Plus Substrate is an acridan-based chemiluminescent and chemifluorescent HRP substrate for western blot detection using X-ray film or CCD- or laser-based imagers. This product is sold as Pierce ECL 2 Substrate (Cat. #PI80196X3) through Fisher Scientific and other channels.

Features of ECL Plus Substrate:

  • Easy to use—can be substituted for the discontinued GE ECL Plus Substrate without any re-optimization
  • Higher sensitivity—detect targets down to the low-picogram level
  • Longer signal duration—sustained light output for as long as 5 hours
  • More imaging options—X-ray, CCD or laser-based imagers
  • More affordable—high quality and performance at a lower price than other suppliers' similar substrates

Pierce ECL Plus Substrate generates acridinium esters when it reacts with HRP. As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film CCD imaging. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers. Pierce ECL Plus Substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. Its broad dynamic range, high sensitivity and long-lasting signal make Pierce ECL Plus Substrate an excellent choice for western blot analysis.

TRUSTED_SUSTAINABILITY

Specifications

Product Type Substrate
Detection Method Chemiluminescence, Fluorescence
Form Liquid
For Use With (Application) Western Blot
Membrane Compatibility Nitrocellulose, PVDF
Product Line Pierce™
Quantity 300 mL Kit
Sensitivity Low picogram to mid-femtogram
Sufficient For 3000 cm2 of membrane
Content And Storage Upon receipt store at 4°C. Product shipped on ice.

For Research Use Only. Not for use in diagnostic procedures.

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