Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. Western Blot Products
  4. Western Blot Substrates and Substrate Kits
  5. Thermo Scientific™ Pierce™ ECL Western Blotting Substrate

Thermo Scientific™ Pierce™ ECL Western Blotting Substrate

Catalog No. PI32109
Change view
Click to view available options
Quantity:
50 mL
250 mL
500 mL
4 x 250 mL
4 x 500 mL
5 product options available for selection
Product selection table with 5 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
PI32109 50 mL
FER32209X4 4 x 250 mL
PI32209 250 mL
FER32106X4 4 x 500 mL
PI32106 500 mL
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 5 options available.
5 options
Catalog No. PI32109 Supplier Thermo Scientific™ Supplier No. 32109
Only null left
Add to Cart
Edge
Add to Cart

Description

Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

Thermo Scientific™ Pierce ECL Western Blotting Substrate is a value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

Pierce ECL Western Blotting Substrate provides reliability and performance equivalent to other standard enhanced chemilumescent (ECL) substrates for detection of horseradish peroxidase (HRP) enzyme activity, yet costs much less. Because the luminol and peroxide reagent-formulations are identical to other commercially available substrate products, one can switch to Pierce ECL without needing to optimize probing conditions or incubation protocols. Simply switch to Pierce ECL and save a bundle.

Highlights:

  • ECL substrate – an enhanced chemiluminescent substrate for detection of horseradish peroxidase (HRP) activity from antibodies and other Western blot probes

  • About half the cost of other ECL Substrates – low overhead and a commitment to customer value enables Thermo Fisher Scientific to make this product available to you at a significantly lower price than other companies (these claims are based on the 2010 U.S. list prices)

  • No optimization required – switching to Pierce ECL substrate from other entry-level ECL substrates does not require optimization or protocol changes

  • A name you can can rely on – count on the strong quality assurance, technical support and reputation behind Thermo Scientific Pierce Protein Research Products

Includes:

Detection Reagent 1 and 2

TRUSTED_SUSTAINABILITY

Specifications

Product Type Substrate
Detection Method Chemiluminescence
Form Liquid
For Use With (Application) Western Blot
Membrane Compatibility Nitrocellulose, PVDF
Product Line Pierce
Quantity 50 mL
Substrate Horseradish Peroxidase
Recommended Antibody Concentrations 2°: 1:1-15K (0.07-1μg/mL), 1°: 1:1K (0.2- 10μg/mL)
Sensitivity Low Picogram
Signal Duration 1 to 2 hr.
Sufficient For 400 sq. cm
Kit Contents Luminol/Enhancer, 25mL, Stable Peroxide Buffer, 25mL
Content And Storage Upon receipt store at 2-8°C. Product is shipped at ambient temperature.
Show More Show Less

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.