Thermo Scientific™ Pierce™ ECL Western Blotting Substrate

Catalog No.	Quantity
PI32109	50 mL
FER32209X4	4 x 250 mL
PI32209	250 mL
FER32106X4	4 x 500 mL
PI32106	500 mL

Catalog No. PI32109 Supplier Thermo Scientific™ Supplier No. 32109

Description

Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

Thermo Scientific Pierce ECL Western Blotting Substrate is a value-priced, entry-level horseradish peroxidase (HRP) substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

Pierce ECL Plus Western Blotting Substrate characteristics include:

• Sensitivity: low picogram
• Stability: 1-hr working solution stability, 1-year kit stability at 4°C
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: 1 to 2 hours
• Recommended primary antibody concentration: 1:1,000–1:5,000 dilution (0.2–10 μg/mL)
• Recommended secondary antibody concentration: 1:1,000–1:15,000 dilution (0.07–1.0 μg/mL)

Pierce ECL Western Blotting Substrate provides reliability and performance equivalent to other standard ECL substrates for detection of HRP enzyme activity. It enables the detection of picogram amounts of antigen and allows for easy detection of HRP using photographic or other imaging methods.

Because the luminol and peroxide reagent formulations are identical to other commercially available substrate products, one can switch to Pierce ECL without needing to optimize probing conditions or incubation protocols. Pierce ECL Western Blotting Substrate provides performance identical to the original Amersham ECL Western Blotting Detection Reagent from GE Healthcare.

Specifications

• Product Type: Substrate
• Detection Method: Chemiluminescence
• Form: Liquid
• For Use With (Application): Western Blot
• Membrane Compatibility: Nitrocellulose, PVDF
• Product Line: Pierce
• Quantity: 50 mL
• Substrate: Horseradish Peroxidase
• Recommended Antibody Concentrations: 2°: 1:1-15K (0.07-1μg/mL), 1°: 1:1K (0.2- 10μg/mL)
• Sensitivity: Low Picogram
• Signal Duration: 1 to 2 hr.
• Sufficient For: 400 sq. cm
• Kit Contents: Luminol/Enhancer, 25 mL, Stable Peroxide Buffer, 25 mL
• Content And Storage: Upon receipt store at 2-8°C. Product is shipped at ambient temperature.

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.