Invitrogen™ Multispecies ERK1/ERK2 In-Cell ELISA Kit, Near Infrared

Catalog No.	Quantity
PI62211	96 Tests

Catalog No. PI62211 Supplier Invitrogen™ Supplier No. 62211

Description

ELISA

The ERK1/2 Multispecies In-Cell ELISA Near Infrared Detection Kits provide a simple and convenient method for quantifying intracellular proteins in whole cells. Principle of the method This kit enables simultaneous detection of post-translational-modified protein (PTM) and the corresponding unmodified protein, or two different targets within the same well. Simultaneously detecting targets within an ELISA well eliminates variability caused by differences in cell plating. The expression levels of the protein(s) are monitored using primary antibodies specific to the targets and corresponding species-specific near-infrared Thermo Scientific DyLight-Conjugated Secondary Antibodies. Relative protein activation or inactivation is determined as a ratio of modified protein levels to the corresponding unmodified protein measured using a modification-specific antibody and an antibody to unmodified protein, respectively. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.

Specifications

• Accession Number: P27361, P28482
• Description: Pierce ERK1/2 Near Infrared In-Cell ELISA Kit
• Conjugate: DyLight™ 680, DyLight™ 800
• Immunoassay Kit Format: Multi-component kit with fluorescent conjugates
• Product Type: ELISA
• Sample Type: Supernatant
• Sufficient For: 96 microplate wells of overnight-cultured cells
• Target Species: Human
• For Use With (Equipment): Fluorescent Microplate Reader
• Gene ID (Entrez): 5594, 5595
• Gene Symbol: ERK,ERK-2,ERK2,ERT1,MAPK2,P42MAPK,PRKM1,PRKM2,p38,p40,p41,p41mapk,p42-MAPK,ERK-1,ERK1,ERT2,HS44KDAP,HUMKER1A,P44ERK1,P44MAPK,PRKM3,p44-ERK1,p44-MAPK
• Kit Contents: Blocking Buffer, 20 x Tris Buffered Saline, Surfact-Amps 20, Surfact-Amps X-100, DyLight® 680 Goat Anti-Mouse IgG (H+L), DyLight 800 Goat Anti-Rabbit IgG (H+L), Thin Plate Seal Assembly, Components included only in the target-specific kits: Antibody #1 and Antibody #2
• Label or Dye: DyLight™ 680, DyLight™ 800
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Supernatant 10,000 cells
• Storage Requirements: 2°C to 8°C
• Target: ERK1/2
• Test Time: 1 hrs 20 mins
• Total Assay Time: 24 hrs