Thermo Scientific™ Pierce™ Fast Western Blot Kit, ECL Substrate

Description

Optimized reagents that shorten the time to perform a typical Western blot from 4 hours to approximately 55 minutes.

Thermo Scientific™ Pierce Fast Western Blot Kit, ECL Substrate contains optimized reagents that shorten the time to perform a typical Western blot from 4 hours down to approximately 55 minutes.

The kit provides all the reagents necessary to complete a Western blot being probed with a mouse or rabbit primary antibody. The protocol requires minimal hands-on time and yields results comparable to classic Western blotting with ECL. The included Pierce ECL Substrate produces a chemiluminescent signal, which is detected using photographic or other imaging methods. Blots can be repeatedly exposed to film to obtain optimal results or stripped of the primary antibody and immunodetection reagents and reprobed.

Highlights:

• Fast – all the sensitivity of Pierce ECL substrate; saves three to four hours over traditional blotting methods

• Convenient – no expensive hardware or extra bench space needed; eliminates membrane clogging issues

• Economical – costs about the same as a classical Western blot; no expensive consumables or extra equipment

• Easy – optimized kits includes all reagents except primary antibody

• Versatile – kits work with both rabbit and mouse primary antibodies

Includes:

Kits contain all reagents to perform Western blots probed with mouse and rabbit primary antibodies, including: Fast Western Antibody Diluent; Fast Western 10X Wash Buffer; Fast Western Optimized HRP Reagent; Pierce ECL Detection Reagent 1; Pierce ECL Detection Reagent 2

Specifications

• Product Type: Western Blot Kit
• Detection Method: Chemiluminescence
• Form: Liquid
• For Use With (Application): Western Blot
• Membrane Compatibility: Nitrocellulose, PVDF
• Product Line: Pierce™
• Quantity: 200 mL Kit
• Conjugate: HRP
• Substrate: Horseradish Peroxidase
• Reactivity: Mouse, Rabbit
• Sufficient For: 5 Mini Blots
• Content And Storage: Sufficient For: 5 mini blots probed with mouse or rabbit primary antibodies
  • Fast Western Antibody Diluent, 100 mL
  • Fast Western 10X Wash Buffer, 50 mL
  • Fast Western Optimized HRP Reagent, 5 mL
  • Pierce ECL Detection Reagent 1, 25 mL
  • Pierce ECL Detection Reagent 2, 25 mL
  
  Store at 4°C.

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.