Thermo Scientific™ Pierce Fast Western Blot Kit, ECL Substrate contains optimized reagents that shorten the time to perform a typical Western blot from 4 hours down to approximately 55 minutes.The kit provides all the reagents necessary to complete a Western blot being probed with a mouse or rabbit primary antibody. The protocol requires minimal hands-on time and yields results comparable to classic Western blotting with ECL. The included Pierce ECL Substrate produces a chemiluminescent signal, which is detected using photographic or other imaging methods. Blots can be repeatedly exposed to film to obtain optimal results or stripped of the primary antibody and immunodetection reagents and reprobed.
Fast – all the sensitivity of Pierce ECL substrate; saves three to four hours over traditional blotting methods
Convenient – no expensive hardware or extra bench space needed; eliminates membrane clogging issues
Economical – costs about the same as a classical Western blot; no expensive consumables or extra equipment
Easy – optimized kits includes all reagents except primary antibody
Versatile – kits work with both rabbit and mouse primary antibodies
Kits contain all reagents to perform Western blots probed with mouse and rabbit primary antibodies, including: Fast Western Antibody Diluent; Fast Western 10X Wash Buffer; Fast Western Optimized HRP Reagent; Pierce ECL Detection Reagent 1; Pierce ECL Detection Reagent 2
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