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  6. 46630

Thermo Scientific™ Krypton™ Fluorescent Protein Stain

Catalog No. 46630
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Quantity:
500 mL

Description

Stain proteins in polyacrylamide gels or transfer membranes for fluorescent detection with popular fluorescence imagers.

Thermo Scientific™ Pierce Krypton Fluorescent Protein Stain enables sensitive fluorescent visualization (520nm excitation; 580nm emission) of proteins separated by polyacrylamide gel electrophoresis or on transfer membranes using popular commercial fluorescence imagers.

This fluorescent protein gel stain is for bright yellow-orange fluorescent detection of proteins separated by 1D or 2D polyacrylamide gel electrophoresis (SDS-PAGE). Krypton Stain uses a protein-binding dye with fluorescence properties (Ex/Em = 520/580nm) suitable for visualization with a variety of standard laser-based and filter-based fluorescence imagers and documentation systems. Proteins are detectable at concentrations greater than 0.25ng per band in a typical mini-gel lane (greater than 2ng for the rapid staining protocol). Krypton Stain provides high signal intensity, exhibits minimal protein-to-protein variation, has an excellent quantitative dynamic range and is compatible with subsequent destaining and analysis by mass spectrometry.

Highlights:

  • Intense fluorescent stain – excitation and emission maxima of 520nm and 580nm, respectively (compatible with 532nm excitation light sources and 580 to 600nm emission filters)

  • Instrument-compatible – effective with popular laser-based and filter-based fluorescence imagers

  • Broad dynamic range – linear quantitative range spans three to four orders of magnitude

  • Sensitive – detect as little as 0.25ng protein with the standard protocol (approx. 2.5 hours); at least as sensitive as fluorescent stains from other suppliers

  • Fast – standard (2.5 hours) and rapid (30 minutes) protocols are significantly faster than procedures with other popular fluorescent stains

  • Versatile – compatible with proteomics workflows involving downstream mass spectrometry

  • Compact – supplied as a 10X solution to save storage space; just dilute with water before use

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Upon receipt store at 4°C.
Detection Location In-Gel Detection
Detection Method Fluorescence
Label or Dye Proprietary Mix
Product Type Protein Stain
Product Line Krypton™
Quantity 500 mL
Target Molecule Protein

For Research Use Only. Not for use in diagnostic procedures.

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