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Thermo Scientific™ 1-Step™ NBT/BCIP Substrate Solution
Description
Thermo Scientific™ Pierce NBT and BCIP Substrates are powders and ready-made solutions of nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate for chromogenic blot and IHC staining with alkaline phosphatase probes.
Highlights:
AP substrate – for detection of alkaline phosphatase (alk-phos) activity on solid media, including nitrocellulose and PVDF membranes and fixed tissue samples
Chromogenic – no special equipment needed for visualization; produces purple-black precipitate that is easy to photograph
Package options – choose individual NBT and BCIP powders, or preformulated, ready-to-use solutions with or without levamisole suppressor
1-Step™ NBT/BCIP
- Ideal for immunoblotting applications
- Minimal assay-to-assay variability
1-Step NBT/BCIP Plus Suppressor
- Ideal for staining tissues assayed with calf intestinal alkaline phosphatase without the interference caused by endogenous phosphatases
- Contains 1mM levamisole to inhibit endogenous phosphatase activity

Specifications
Specifications
Description | Pierce 1-Step NBT/BCIP Substrate Solution |
Product Type | Substrate |
Detection Method | Colorimetric |
Form | Liquid |
Membrane Compatibility | Nitrocellulose, PVDF |
Product Line | 1-Step™ |
Quantity | 250 mL |
Substrate | Alkaline Phosphatase |
Reactivity | Alkaline Phosphatase |
Content And Storage | Upon receipt store product at 4°C. Product is shipped at ambient temperature. |
For Research Use Only. Not for use in diagnostic procedures.
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