Thermo Scientific NHS-Azide
Activated azido compounds to derivatize proteins or amine surfaces for selective conjugation, detection or purification by phosphine-modified probes.
Manufacturer: Thermo Scientific 88902
Thermo Scientific Pierce NHS-Azide reagents are amine-reactive compounds that can be used to derivatize primary amines of proteins or amine-coated polymer surfaces for ligation to phosphine-modified molecules.
These NHS-ester compounds react to form covalent bonds with primary amines (e.g., side chain of lysine residues or aminosilane-coated surfaces). The azide (N3) group reacts with phosphine-labeled molecules by a mechanism known as Staudinger chemistry, enabling efficient and specific conjugation of derivatized molecules in biological samples. In combination with different phosphine-activated compounds, these NHS-Azide reagents facilitate a variety of labeling and crosslinking experimental strategies for investigating protein interactions and cellular pathways.
Soluble reagents easily dissolve in water-miscible solvents for subsequent dilution in aqueous reaction mixtures with cell lysates and other biological samples
Compatible reaction chemistry occurs effectively in simple buffer conditions; requires no accessory reagents such as copper or reducing agents
Specific NHS esters are specific for covalent attachment to primary amines (side chain of lysine or N-terminus of polypeptides)
Chemoselective azide and phosphine groups do not react or interfere with components of biological samples but conjugate to one another with high efficiency
Pegylated choose the very short NHS-Azide or one of the two varieties whose spacer arms contain medium (4-unit) or long (12-unit) polyethylene glycol (PEG) groups for increased solubility and reach
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|DMSO (Dimethylsulfoxide), DMF (Dimethylformamide)|
|Upon receipt store at -20°C protected from light and moisture. Product is shipped at ambient temperature.|
|Amine, Azide, NHS Ester|