Thermo Scientific™ SDAD (NHS-SS-Diazirine) (succinimidyl 2-((4,4'-azipentanamido)ethyl)-1,3'-dithiopropionate)

Description

Very short to mid-length (3.9 to 13.5A), amine-to-anything crosslinkers; NHS-ester and diazirine reactive groups; photoactivate with UV light (Amax 330 to 370nm).

Thermo Scientific™ Pierce NHS-Diazirine Crosslinkers combine proven NHS-ester and diazirine-based photoreaction chemistries to conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation.

The succinimidyl-ester diazirine (SDA) reagents are a new class of crosslinkers that combine proven amine-reactive chemistry with an innovative and efficient diazirine-based photochemistry for conjugating amine-containing molecules to nearly any other functional group. The SDA crosslinkers include six compounds differing in spacer arm lengths, ability to cleave the crosslinked proteins, and presence or absence of a charged group for membrane permeability. Protein crosslinking is an important technique used to understand protein structure and to stabilize protein-protein interactions. Thermo Scientific SDA Reagents extend the efficiency and range of interactions that can be explored by this approach.

Highlights:

• Versatile - different amine-reactive diazirine derivatives capture specific intracellular or cell-surface protein interactions

• Controllable - two-step chemical crosslinking is activated using common laboratory UV lamps

• Reversible - disulfide-containing spacers enable cleavage using reducing agents

• Easy - reagents are photo-stable under typical laboratory lighting conditions so there is no need to perform experiments in the dark

• Better than aryl azides - diazirine photo-reactive group has better photostability and is more efficiently activated by long-wave UV light

Specifications

• Chemical Reactivity: Amine-Nonselective
• Cleavable: By Thiols
• Description: SDAD
• Molecular Weight (g/mol): 388.46
• PEGylated: No
• Spacer Arm Length: 13.5 Å
• Content And Storage: Upon receipt store product at 4°C protected from light and moisture.
• Cell Permeability: Yes
• Shipping Condition: Ambient
• Product Line: Pierce™
• Labeling Method: Chemical Labeling
• Crosslinker Type: Heterobifunctional
• Reactive Moiety: NHS Ester, Diazirine
• Spacer: Medium (10 to 30 Å)
• Form: Solid
• Quantity: 50 mg
• Solubility: DMF, DMSO
• Format: Standard
• Water Soluble: No
• Product Type: Crosslinker

For Research Use Only. Not for use in diagnostic procedures.