Thermo Scientific™ Sulfo-SDAD (Sulfo-NHS-SS-Diazirine) (sulfosuccinimidyl 2-((4,4'-azipentanamido)ethyl)-1,3'-dithiopropionate)

Catalog No.	Quantity
PI26175	50 mg

Catalog No. PI26175 Supplier Thermo Scientific™ Supplier No. 26175

Description

Very short to mid-length (3.9 to 13.5A), amine-to-anything crosslinkers; NHS-ester and diazirine reactive groups; photoactivate with UV light (Amax 330 to 370nm).

Thermo Scientific™ Pierce NHS-Diazirine Crosslinkers combine proven NHS-ester and diazirine-based photoreaction chemistries to conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation.

The succinimidyl-ester diazirine (SDA) reagents are a new class of crosslinkers that combine proven amine-reactive chemistry with an innovative and efficient diazirine-based photochemistry for conjugating amine-containing molecules to nearly any other functional group. The SDA crosslinkers include six compounds differing in spacer arm lengths, ability to cleave the crosslinked proteins, and presence or absence of a charged group for membrane permeability. Protein crosslinking is an important technique used to understand protein structure and to stabilize protein-protein interactions. Thermo Scientific SDA Reagents extend the efficiency and range of interactions that can be explored by this approach.

Highlights:

• Versatile - different amine-reactive diazirine derivatives capture specific intracellular or cell-surface protein interactions

• Controllable - two-step chemical crosslinking is activated using common laboratory UV lamps

• Reversible - disulfide-containing spacers enable cleavage using reducing agents

• Easy - reagents are photo-stable under typical laboratory lighting conditions so there is no need to perform experiments in the dark

• Better than aryl azides - diazirine photo-reactive group has better photostability and is more efficiently activated by long-wave UV light

Specifications

• Chemical Reactivity: Amine-Nonselective
• Cleavable: By Thiols
• Description: Sulfo-SDAD
• Molecular Weight (g/mol): 490.51
• PEGylated: No
• Spacer Arm Length: 13.5 Å
• Content And Storage: Upon receipt store product at 4°C protected from light and moisture.
• Cell Permeability: No
• Shipping Condition: Ambient
• Product Line: Pierce
• Labeling Method: Chemical Labeling
• Crosslinker Type: Heterobifunctional
• Reactive Moiety: Diazirine, Sulfo-NHS Ester
• Spacer: Medium (10 to 30 Å)
• Form: Solid
• Quantity: 50 mg
• Solubility: Water
• Format: Standard
• Water Soluble: Yes
• Product Type: Crosslinker

Frequently Asked Questions (FAQs)

What are the options for crosslinking proteins to nucleic acids, and what are the differences between them?

We offer the following crosslinkers for protein to nucleic acids: formaldehyde (Cat. No. 28906), SPB (Cat. No. 23013), SDAD (Cat. No. 26169), sulfo-SDAD (Cat. No. 26175), and sulfo-SBED (Cat. No. A39260).
Formaldehyde can be used for DNA-DNA crosslinking as well as DNA-protein crosslinking. While it is good for crosslinking proteins that are in direct contact with DNA, formaldehyde cannot link proteins that may be bound in a complex with other proteins but are not in direct contact with DNA. EGS or DSG can crosslink proteins to other proteins that are in direct contact with DNA and crosslinked to DNA by formaldehyde.
SPB, Sulfo-SDAD, and Sulfo-SBED are all photoreactive crosslinkers. SPB is an NHS-ester and psoralen heterobifunctional crosslinker that conjugates primary amines on proteins to DNA via photo-activated intercalation of psoralen to pyrimidine bases. The psoralen tricyclic planar ring system intercalates into double-stranded, and to a lesser extent, single-stranded DNA and RNA. The photoreactive psoralen group provides more selective covalent binding to nucleic acids than either phenyl azide or diazerine linkers, which makes SPB the best choice. The diazerine on sulfo-SDAD and the phenyl azide on sulfo-SBED are not selective for nucleic acids and can crosslink other biomolecules in the same besides any protein-DNA interactions. Sulfo-SBED involves biotin transfer rather than actual linking.

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.