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Thermo Scientific™ NHS-Phosphine
Description
Thermo Scientific™ Pierce NHS-Phosphine and Sulfo-NHS-Phosphine are amine-reactive compounds that can be used to derivatize primary amines of proteins or amine-coated polymer surfaces for ligation to azide-tagged molecules.
Highlights:
Versatile – Sulfo-NHS-Phosphine easily dissolves in water-miscible solvents for subsequent dilution in aqueous reaction mixtures for azide conjugation; NHS-Phosphine can be used for amine surface modification in organic solvents
Compatible – reaction chemistry occurs effectively in simple buffer conditions; requires no accessory reagents such as copper or reducing agents
Specific – NHS esters are specific for covalent attachment to primary amines (side chain of lysine or N-terminus of polypeptides)
Chemoselective – azide and phosphine groups do not react or interfere with components of biological samples but conjugate to one another with high efficiency

Specifications
Specifications
Chemical Reactivity | Amine-Azide |
Cleavable | No |
Description | NHS-Phosphine |
Molecular Weight (g/mol) | 461.4 |
PEGylated | No |
Spacer Arm Length | 5.4 Å |
Content And Storage | Upon receipt store at -20°C protected from light and moisture. |
Cell Permeability | Not determined |
Shipping Condition | Ambient |
Product Line | Pierce™ |
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Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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