Thermo Scientific™ NHS-Phosphine

Description

Derivatize proteins or amine surfaces for selective conjugation, detection or purification of azide-tagged targets with these activated phosphine compounds.

Thermo Scientific™ Pierce NHS-Phosphine and Sulfo-NHS-Phosphine are amine-reactive compounds that can be used to derivatize primary amines of proteins or amine-coated polymer surfaces for ligation to azide-tagged molecules.

These NHS-ester compounds react to form covalent bonds with primary amines (e.g., side chain of lysine residues or aminosilane-coated surfaces). The phosphine groups (PPh2) react with azide-labeled molecules by a mechanism known as Staudinger chemistry, enabling efficient and specific conjugation of derivatized molecules in biological samples. In combination with different azide-tagged compounds, these NHS-Phosphine reagents facilitate a variety of labeling and crosslinking experimental strategies for investigating protein interactions and cellular pathways.

Highlights:

• Versatile – Sulfo-NHS-Phosphine easily dissolves in water-miscible solvents for subsequent dilution in aqueous reaction mixtures for azide conjugation; NHS-Phosphine can be used for amine surface modification in organic solvents

• Compatible – reaction chemistry occurs effectively in simple buffer conditions; requires no accessory reagents such as copper or reducing agents

• Specific – NHS esters are specific for covalent attachment to primary amines (side chain of lysine or N-terminus of polypeptides)

• Chemoselective – azide and phosphine groups do not react or interfere with components of biological samples but conjugate to one another with high efficiency

Specifications

• Chemical Reactivity: Amine-Azide
• Cleavable: No
• Description: NHS-Phosphine
• Molecular Weight (g/mol): 461.4
• PEGylated: No
• Spacer Arm Length: 5.4 Å
• Content And Storage: Upon receipt store at -20°C protected from light and moisture.
• Cell Permeability: Not determined
• Shipping Condition: Ambient
• Product Line: Pierce™
• Labeling Method: Chemical Labeling
• Crosslinker Type: Heterobifunctional
• Reactive Moiety: NHS Ester, Phosphine
• Spacer: Short (<10 Å)
• Form: Powder
• Quantity: 10 mg
• Solubility: DMF, DMSO
• Format: Standard
• Water Soluble: No
• Product Type: Crosslinker

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.