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Thermo Scientific™ ONPG (o-nitrophenyl-β-D-galactopyranoside)
Description
Thermo Scientific™ Pierce ONPG Substrate is the preferred colorimetric substrate for ELISA applications involving β-galactosidase (β-Gal) as the reporter enzyme.
ONPG (ω-nitrophenyl-β-D-galactopyranoside) is the preferred colorimetric substrate for ELISA applications involving β-galactosidase (β-Gal) reporter enzyme. The enzyme-substrate reaction yields a yellow product with an absorbance maximum at 420nm but whose intensity is also very high at 405nm (matching a common plate reader filter set).
Highlights:
- Product formed is completely soluble
- High extinction coefficient at 405nm
- Substrate yields a yellow product that is easily detectable in the visual range after stopping the reaction with 1M sodium carbonate
Specifications
Specifications
| Product Type | ELISA Substrate |
| Quantity | 5 g |
| Detection Method | Colorimetric |
| Substrate Properties | Chemical Substrate |
| Substrate Type | Beta-Gal |
| Content And Storage | Upon receipt store at -20°C protected from moisture and light. Product is shipped at ambient temperature. |
Safety and Handling
WARNING: Cancer - www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
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