Thermo Scientific™ Pierce™ Protein-Free Blocking Buffer

Catalog No.	Quantity	Chemical Name or Material
PI37572	1 L	Protein-Free (PBS)
PI37584	100 mL	Protein-Free (PBS)
PI37585	100 mL	Protein-Free (TBS)
PI37570	1 L	Protein-Free (TBS)
PI37573	1 L	Protein-Free T20 (PBS)
PI37571	1 L	Protein-Free T20 (TBS)

Catalog No. PI37572 Supplier Thermo Scientific™ Supplier No. 37572

Description

Pierce Protein-Free Blocking Buffer is a non-protein compound that provides effective blocking for membrane-based and plate-based protein detection methods, resulting in extremely low background.

Pierce Protein-Free Blocking Buffer is a non-protein compound that provides effective blocking for membrane-based and plate-based protein detection methods, resulting in extremely low background. It is supplied in a convenient, ready-to-use format.

Features of Protein-Free Blocking Buffer include:

• Protein-free blocker—minimizes or eliminates cross-reactivity associated with protein-based blocking buffers
• Applications—compatible with western blotting (membranes), ELISA (microplates), and arrays (coated glass slides)
• Streptavidin-friendly—free of biotin; no interference with avidin-biotin detection systems
• High-performance—optimized and validated in many protein methods to provide high signal-to-noise ratio (i.e., no quenching of specific binding and signal but eliminating nonspecific binding and background)

Traditional blocking buffers contain proteins that can cross-react in certain immunodetection systems, resulting in high background and reduced signal. Pierce Protein-Free Blocking Buffers are devoid of protein while remaining highly effective at blocking plates, membranes, and other surfaces for ELISA, western blotting, glass slide arrays, and other applications.

Specifications

• Chemical Name or Material: Protein-Free (PBS)
• Concentration: 1X
• For Use With (Application): ELISA
• Physical Form: Liquid
• Product Line: Pierce
• Quantity: 1 L

Frequently Asked Questions (FAQs)

How can I reduce background bands in my Western blot?

Optimize the concentration of primary and secondary antibodies. In some cases, increasing the concentration of blocking agent (BSA or non-fat dry milk) or usiing an alternative blocking solution such as Starting Block or SuperBlock may reduce background signal. After incubation with the primary antibody, wash at least 2 times with TBST (include 0.5 M NaCl in one or more of the wash steps). Avoid Nonidet P40 or Triton X-100 in buffers because protein detection is decreased when these detergents are used.

Is the Pierce Protein-Free Blocking Buffer (Cat. No. 37571) animal-origin free (AOF)?

Yes, it is animal-origin free (AOF).