Thermo Scientific™ Pierce™ SDS-PAGE Sample Prep Kit

Description

Quickly and easily remove high levels of salts, denaturants, detergents and other buffer components that interfere with polyacrylamide gel electrophoresis.

Thermo Scientific™ Pierce SDS-PAGE Sample Prep Kit quickly and easily removes high levels of salts, denaturants, detergents and other buffer components that interfere with polyacrylamide gel electrophoresis of proteins.

The SDS-PAGE Sample Prep Kit provides a simple and effective method for concentrating samples while removing chemicals that interfere with SDS-PAGE analysis, such as acids and bases, detergents, guanidine and ammonium sulfate. The proprietary PAGE-prep Resin binds protein in the presence of dimethylsulfoxide (DMSO). Interfering contaminants are then removed by washing the protein-bound resin in an easy-to-use spin cup format. Proteins are eluted in a buffer compatible with the Pierce BCA Protein Assay, allowing quantitation of a portion of the processed sample. The eluted proteins can then be combined with sample buffer and analyzed on the SDS-PAGE system of choice.

Highlights:

• Eliminates gel artifacts caused by incompatible contaminants – removes dyes, reducing agents, detergents, sugars, glycerol, guanidine, urea and ammonium sulfate to provide reproducible results by SDS-PAGE analysis

• Compatible with the BCA protein assay – allows protein quantitation of the processed sample before gel-loading

• Enriches dilute protein solutions – concentrates protein samples for SDS-PAGE analysis by six-fold in less than 20 minutes

• Powerful and flexible – process 2 to 350μL of sample containing up to 70μg of protein; recover 75 to 85% of the total protein in 50μL of elution buffer

• Convenient, complete kit – includes PAGEprep Resin, DMSO, spin cups and sample loading buffer

  • Includes:

    PAGEprep resin slurry, DMSO solution, elution buffer, sample buffer, spin cups and collection tubes

Specifications

• Content And Storage: Upon receipt store kit at room temperature.
• Buffer: Sample Loading Buffers
• Gel Type: SDS-PAGE
• Product Type: SDS-PAGE Sample Prep Kit
• Gel Compatibility: SDS-PAGE Gels
• Product Line: Pierce™
• Quantity: 50 reactions

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.