Thermo Scientific™ Stop Solution for TMB Substrates 

Stop TMB-based ELISA detection with this 0.16M sulfuric acid for use with the ELISA substrate 3,3',5,5' - tetramethylbenzidine (TMB).

Manufacturer:  Thermo Scientific™ N600

Catalog No. PIN600

Description

Thermo Scientific™ Pierce Stop Solution for TMB is 0.16M sulfuric acid for use with the ELISA substrate 3,3',5,5' - tetramethylbenzidine (TMB).

TMB Stop Solution is 0.16M sulfuric acid for use with the ELISA substrate 3,3',5,5'-tetramethylbenzidine (TMB). Addition of sulfuric acid stop solution changes the color from blue to yellow, stabilizing the color development to enable accurate measurement of the intensity at 450nm using a spectrophotometer or plate reader.

• Size: 55mL

• Sufficient For: five 96-well microplates

• Application: ELISA

Instructions: Following TMB incubation, add an amount of stop solution equal to the amount of TMB substrate added to each well of the ELISA plate (typically 50 to 100µL per well). Use at full strength.

This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

Specifications

• Description: Pierce Stop Solution for TMB
• Storage Requirements: Room Temperature or 2°C to 8°C
• Quantity: 55 mL
• Form: Liquid
• Validated Application: ELISA

• Formulation: 0.16M sulfuric acid
• Sufficient For: Stopping color-development of TMB substrate in approx. 5 standard plates
• Content And Storage: Store at 2–8°C.
• Reagent Type: Stop Solution