Thermo Scientific™ Pierce™ Fast Western Blot Kits, SuperSignal™ West Dura, Mouse

For femtogram-level detection and Western blot development less than 1 hour after gel transfer.

Supplier:  Thermo Scientific™ 35070

Catalog No. PI35070

Description

Thermo Scientific™ Pierce Fast Western Blot Kit, SuperSignal West Dura Substrate contains optimized reagents and protocol that allows femtogram levels of detection and shortens typical Western blot development to less than 1 hour after the gel transfer.

The Fast Western Blot Kit protocol is a quick and efficient method for developing Western blots without the need to purchase expensive instrumentation and consumables required by other rapid Western blotting systems. These complete Pierce Fast Western Blot Kits require minimal hands-on time and yield results comparable to classic Western blotting with SuperSignal West Dura Chemiluminescent Substrate. Like other Western blotting methods, the included SuperSignal West Dura Substrate produces a chemiluminescent signal that is detectable using photographic or other imaging methods. The developed blots can be repeatedly exposed and stripped of the primary antibody and reprobed.

Highlights:

• Fast – femtogram levels of detection in about one hour

• Convenient – no expensive hardware or vacuum required; no clogging issues

• Simple – optimized protocol makes Western blot analysis easier than ever

• Economical – no additional consumables or extra equipment to purchase

• Stable – all kit components are stable for one year stored at 4°C

• Easy – complete kits contain all components needed to block, probe and develop a blot with any standard mouse or rabbit primary antibody

Compatible with:

Pierce Membranes and Filter Paper; CL-Xposure™ Film for Western blotting; Pierce Western Blot Signal Enhancer; Restore Plus™ Western Blot Stripping Buffer

Specifications

• Description: Pierce Fast Western Blot Kit, SuperSignal West Dura Substrate, Mouse
• Detection Method: Western Blot
• For Use With (Application): Western Blot
• Product Line: Pierce™, SuperSignal™
• Conjugate: HRP
• Reactivity: Mouse
• Sufficient For: 10 western blots (800 sq. cm of membrane)

• Product Type: Western Blot Kit
• Form: Liquid
• Membrane Compatibility: Nitrocellulose, PVDF
• Quantity: 400 ml
• Substrate: Luminol
• Sensitivity: Femtogram-level
• Content And Storage: Sufficient For: 10 mini blots probed with mouse primary antibodies
  • Fast Western Antibody Diluent, 200 mL
  • Fast Western 10X Wash Buffer, 100 mL
  • Fast Western Mouse Optimized HRP Reagent, Dura, 10 mL
  • SuperSignal West Dura Luminol/Enhancer Solution, 50 mL
  • SuperSignal West Stable Peroxide Solution, 50 mL
  
  Store kit at 4°C.