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Pig anti-Human IgE Fc Secondary Antibody, Novus Biologicals™
SDP

Catalog No. NBP269114 Shop All R&D Systems Products
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10 mg
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Catalog No. Quantity
NBP2-69114 10 mg
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Catalog No. NBP2-69114 Supplier Novus Biologicals Supplier No. NBP269114
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Description

Pig Polyclonal Antibody

IgE Fc Polyclonal specifically detects IgE Fc in Human samples. It is validated for ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunoblotting, Dot Blot.

Specifications

Antigen IgE Fc
Applications ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoblot, Dot Blot
Classification Polyclonal
Concentration LYOPH
Conjugate Unconjugated
Dilution ELISA 1:100-1:2000, Immunocytochemistry/Immunofluorescence 1:10 - 1:500, Immunohistochemistry-Paraffin 1:10 - 1:500, Immunoblotting 1:100 - 1:2000, Dot Blot 1:100-1:2000
Formulation Lyophilized from PBS (pH 7.2) with No Preservative
Host Species Pig
Immunogen Purified homogenous IgE isolated from human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Purification Method Ion exchange chromatography
Quantity 10 mg
Primary or Secondary Secondary
Test Specificity The reactivity of the antiserum is directed to the Fc subunits of the IgE molecule which represents strict isotype (class) specificity. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against appropriate concentrations of IgE, a single precipitin line is obtained which shows a reaction of full identity with the precipitin line obtained with the used immunogen. It does not react with human IgG, including all subclasses, IgA, IgM and IgG/Fab or any non-Ig protein in human serum.
Reconstitution Reconstitute by by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20C.
Target Species Human
Content And Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Form Lyophilized
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