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Pig anti-Human IgG Fc Secondary Antibody, FITC, Novus Biologicals™
SDP

Catalog No. NBP269488 Shop All R&D Systems Products
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NBP2-69488 1 mL
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Catalog No. NBP2-69488 Supplier Novus Biologicals Supplier No. NBP269488
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Pig Polyclonal Antibody

IgG Fc Polyclonal antibody specifically detects IgG Fc in Human samples. It is validated for ELISA, Immunocytochemistry, Immunofluorescence, Immunoblot, Dot Blot.

Specifications

Antigen IgG Fc
Applications ELISA, Immunocytochemistry, Immunofluorescence, Immunoblot, Dot Blot
Classification Polyclonal
Concentration LYOPH
Conjugate FITC
Dilution ELISA 1:100-1:2000, Immunocytochemistry/Immunofluorescence 1:10 - 1:500, Immunoblotting 1:100 - 1:2000, Dot Blot 1:100-1:2000
Formulation Lyophilized from PBS (pH 7.2) with No Preservative
Host Species Pig
Immunogen Purified normal IgG isolated from pooled human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Purification Method Ion exchange chromatography
Quantity 1 mL
Primary or Secondary Secondary
Test Specificity The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against normal human plasma and serum, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgG. No reaction is obtained with purified IgA, IgM and IgG/Fab fragments.
Reconstitution Reconstitute by by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20C.
Target Species Human
Content And Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Form Lyophilized
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