Pig anti-Human IgG Fc Secondary Antibody, Novus Biologicals™

Catalog No.	Quantity
NBP2-69484	10 mg

Catalog No. NBP2-69484 Supplier Novus Biologicals Supplier No. NBP269484

Description

Pig Polyclonal Antibody

IgG Fc Polyclonal antibody specifically detects IgG Fc in Human samples. It is validated for ELISA, Immunocytochemistry, Immunofluorescence, Immunoblot, Dot Blot.

Specifications

• Antigen: IgG Fc
• Applications: ELISA, Immunocytochemistry, Immunofluorescence, Immunoblot, Dot Blot
• Classification: Polyclonal
• Concentration: LYOPH
• Conjugate: Unconjugated
• Dilution: ELISA 1:100-1:2000, Immunocytochemistry/Immunofluorescence 1:10 - 1:500, Immunoblotting 1:100 - 1:2000, Dot Blot 1:100-1:2000
• Formulation: Lyophilized from PBS (pH 7.2) with No Preservative
• Host Species: Pig
• Immunogen: Purified normal IgG isolated from pooled human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
• Purification Method: Ion exchange chromatography
• Quantity: 10 mg
• Primary or Secondary: Secondary
• Test Specificity: The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against normal human plasma and serum, a characteristic precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgG. No reaction is obtained with purified IgA, IgM and IgG/Fab fragments or any other serum protein.
• Reconstitution: Reconstitute by by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20C.
• Target Species: Human
• Content And Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Form: Lyophilized