Pig anti-Human IgG Fc Secondary Antibody, Novus Biologicals™
Pig Polyclonal Antibody
Supplier: Novus Biologicals NBP269484
DescriptionIgG Fc Polyclonal antibody specifically detects IgG Fc in Human samples. It is validated for ELISA, Immunocytochemistry, Immunofluorescence, Immunoblot, Dot Blot.
|Lyophilized from PBS (pH 7.2) with No Preservative|
|Purified normal IgG isolated from pooled human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.|
|The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against normal human plasma and serum, a characteristic precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgG. No reaction is obtained with purified IgA, IgM and IgG/Fab fragments or any other serum protein.|
|ELISA, Immunocytochemistry, Immunofluorescence, Immunoblot, Dot Blot|
|ELISA 1:100-1:2000, Immunocytochemistry/Immunofluorescence 1:10 - 1:500, Immunoblotting 1:100 - 1:2000, Dot Blot 1:100-1:2000|
|Ion exchange chromatography|
|Reconstitute by by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20C.|
|Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.|
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