Pig anti-Human IgG (H+L) Secondary Antibody, FITC, Novus Biologicals™

Pig Polyclonal Antibody

Supplier:  Novus Biologicals NBP269399

Catalog No. NBP2-69399

Description

IgG (H+L) Polyclonal specifically detects IgG (H+L) in Human samples. It is validated for ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunoblotting, Dot Blot.

Specifications

• Antigen: IgG (H+L)
• Classification: Polyclonal
• Conjugate: FITC
• Formulation: Lyophilized from PBS (pH 7.2) with No Preservative
• Immunogen: Purified normal IgG isolated from pooled human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
• Quantity: 2 mL
• Primary or Secondary: Secondary
• Reconstitution: Reconstitute by by adding 2 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20C.
• Content And Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Applications: ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoblot, Dot Blot
• Concentration: LYOPH
• Dilution: ELISA 1:100-1:2000, Immunocytochemistry/Immunofluorescence 1:10 - 1:500, Immunohistochemistry-Paraffin 1:10 - 1:500, Immunoblotting 1:100 - 1:2000, Dot Blot 1:100-1:2000
• Host Species: Pig
• Purification Method: Ion exchange chromatography
• Research Discipline: Adaptive Immunity, Immunology
• Test Specificity: The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in human serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
• Target Species: Human
• Form: Lyophilized