Pig anti-Human IgG (H+L) Secondary Antibody, Novus Biologicals™
Pig Polyclonal Antibody
Supplier: Novus Biologicals NBP269397
DescriptionIgG (H+L) Polyclonal specifically detects IgG (H+L) in Human samples. It is validated for ELISA, Immunocytochemistry/Immunofluorescence, Immunoblotting, Dot Blot.
|Lyophilized from PBS (pH 7.2) with No Preservative|
|Purified normal IgG isolated from pooled human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.|
|Reconstitute by by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20C.|
|Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.|
|ELISA, Immunocytochemistry, Immunofluorescence, Immunoblot, Dot Blot|
|ELISA 1:100-1:2000, Immunocytochemistry/Immunofluorescence 1:10 - 1:500, Immunoblotting 1:100 - 1:2000, Dot Blot 1:100-1:2000|
|Ion exchange chromatography|
|Adaptive Immunity, Immunology|
|The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in human serum, as tested by immunoelectrophoresis and double radial immunodiffusion.|
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