Pig anti-Human Kappa Light Chain Secondary Antibody, Novus Biologicals™
Pig Polyclonal Antibody
Supplier: Novus Biologicals NBP269238
DescriptionKappa Light Chain Polyclonal antibody specifically detects Kappa Light Chain in Human samples. It is validated for ELISA, Immunocytochemistry, Immunofluorescence, Immunoblot, Dot Blot.
|Kappa Light Chain|
|Lyophilized from PBS (pH 7.2) with No Preservative|
|A pool of purified Bence Jones kappa proteins isolated from human urine. Freund's complete adjuvant is used in the first step of the immunization procedure.|
|Reconstitute by by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20C.|
|Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.|
|ELISA, Immunocytochemistry, Immunofluorescence, Immunoblot, Dot Blot|
|ELISA 1:100-1:2000, Immunocytochemistry/Immunofluorescence 1:10 - 1:500, Immunoblotting 1:100 - 1:2000, Dot Blot 1:100-1:2000|
|HCAK1, IGKCD, immunoglobulin kappa constant, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809|
|Ion exchange chromatography|
|The reactivity of the antiserum is directed to the surface and hidden determinants of Ig kappa light chain. In immunoelectrophoresis this antiserum is reacting with polyclonal and monoclonal immunoglobulins of the kappa type, Bence Jones proteins as well as free light chains of the kappa type. This antiserum does not react with any other protein of human serum or plasma.|
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