Invitrogen™ PKA alpha Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA517626

Catalog No. PIPA517626

Description

Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay. It is not recommended to aliquot this antibody.

Protein kinase A (PKA, cAMP-dependent protein kinase) is a key element of a ubiquitous signaling pathway important in the cell cycle, cellular communication, memory formation and behavior. PKA is composed of two catalytic (PKAc; Protein Kinase A catalytic subunit) and two regulatory subunits (PKAr). Upon binding cAMP, the complex dissociates to PKAr dimer and two activated PKAc ser/thr protein kinase catalytic monomers. The released PKAc can translocate into the nucleus and exert a regulatory role in the activation of multiple nuclear hormone receptors. However, PKAc-mediated activation of tonicity-dependent gene expression is cAMP independent. Humans express three types of PKAc subunit - PKAc alpha is present in most human tissues, PKAc beta and gamma are tissue-specific, the later is found in testes.

Specifications

• Antigen: PKA alpha
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: PRKACA
• Gene Alias: alternatively spliced; C(s); Calpha; C-alpha-2; C-alpha-S; cAMP-dependent protein kinase catalytic subunit alpha; cAMP-dependent protein kinase catalytic subunit alpha, isoform 1; cAMP-dependent protein kinase catalytic subunit C alpha; cAMP-dependent protein kinase catalytic subunit Calpha1; Cs-PKA; MGC102831; MGC48865; PKA; PKA C-alpha; PKA catalytic subunit alpha; pkac; Pkaca; PKCA1; PKCD; PPNAD4; PRKACA; protein kinase; protein kinase A; protein kinase A alpha; protein kinase A catalytic subunit; protein kinase cAMP-activated catalytic subunit alpha; protein kinase, cAMP dependent, catalytic, alpha; protein kinase, cAMP-dependent, alpha catalytic subunit; protein kinase, cAMP-dependent, catalytic, alpha; sperm cAMP-dependent protein kinase catalytic subunit; sperm cAMP-dependent protein kinase catalytic subunit Cs
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 18747, 25636, 5566
• Content And Storage: -20°C
• Form: Liquid

• Applications: Flow Cytometry, Immunoprecipitation, Western Blot, Immunocytochemistry
• Concentration: 24 μg/mL
• Formulation: 0.01M HEPES with 100 μg/mL BSA, 50% glycerol, 0.15M NaCl and no preservative; pH 7.5
• Gene Accession No.: P05132, P17612, P27791
• Gene Symbols: PRKACA
• Immunogen: Synthetic peptide corresponding to the carboxy terminal sequence of human PKA C-a.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov