Invitrogen™ PKC alpha Polyclonal Antibody

Catalog No.	Quantity
PIPA594941	100 μg

Catalog No. PIPA594941 Supplier Invitrogen™ Supplier No. PA594941

Description

Rabbit Polyclonal Antibody

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 μg/mL. Positive Control - WB: human U87 whole cell, human HEL whole cell, rat brain tissue, mouse brain tissue. IHC: Human Mammary Cancer tissue. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

The PKC family of serine/threonine kinases, including PRKCA (PKC alpha), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent. Protein kinase C (PKC) can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.

Specifications

• Antigen: PKC alpha
• Applications: Immunohistochemistry (Paraffin), Western Blot
• Classification: Polyclonal
• Concentration: 500 μg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 5mg BSA and 0.05mg thimerosal, 0.05mg sodium azide
• Gene: PRKCA
• Gene Accession No.: P05696, P17252, P20444
• Gene Alias: AAG6; aging-associated gene 6; AI875142; EC 2.7.11.13; FHC; FTH; FTHL6; im:7139045; kinase PKC-alpha; KPCA; MGC104426; PIG15; PKC III; Pkca; PKC-A; PKC-alpha; PKC-III; PKRCA; PLIF; PRKACA; Prkca; prkcaa; protein kinase C; protein kinase C alpha; protein kinase C alpha type; protein kinase C alpha type; protein kinase C, alpha, a; protein kinase C, alpha; protein kinase C, alpha, a; protein kinase c-alpha; si:dkey-8l19.1; Unknown (protein for IMAGE:7139045); unnamed protein product
• Gene Symbols: PRKCA
• Host Species: Rabbit
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human PRKCA (153-182aa MDHTEKRGRIYLKAEVADEKLHVTVRDAKN).
• Purification Method: Affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 18750, 24680, 5578
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Lyophilized
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov